Department of Thyroid Breast Surgery, The Third Xiangya Hospital of Central South University, Yuelu district, Changsha, Hunan province 410013, PR China.
Department of Nursing, The Third Xiangya Hospital of Central South University, Yuelu district, Changsha, Hunan province 410013, PR China.
J Photochem Photobiol B. 2019 Mar;192:68-73. doi: 10.1016/j.jphotobiol.2018.12.024. Epub 2019 Jan 7.
The purpose of this report was to progress the advancement of nanotechnology combined with near-infrared (NIR) laser-mediated treatment for enhancing efficiency against breast cancer (MCF-7) cells. The physic-chemical interactions and properties of the surface improved nano-Graphene oxide (nGO) sheets with Copper Sulfide (CuS) quantum dots were analyzed by selected exclusive analytical methods. The cytotoxicity effect of nGO was investigated by various in vitro assays. The surface activated nGO was exhibited its maximum absorption peak at 233 nm. The XRD analysis shows that the intensity of graphite was decreased and new peak arises around 2Ѳ=11.4o with an interlayer distance of 0.728 to 0.828 nm. In addition, microscopic studies demonstrated that the synthesized nGO was found as a transparent layer with the crispy structure as well as few layers appear as nanoflakes. The cell toxicity enhancement on MCF-7 breast cancer cells was highly influenced by the concentration of CuSQDs loaded nGO sheets with an assessed IC50 value was 100 μg/mL. The fluorescence microscopic visualization was confirmed the cells apoptotic morphological variations and cell death in CuSQDs-nGO treated breast cancer (MCF-7) cell line. Furthermore, the augmented level of Caspase-3, ROS and LDH activities of cancer cells were exhibited after CuSQDs-nGO treatment. Therefore, the present detailed biological investigations demonstrated that NIR-combined with surface activated CuSQDs-nGO presented a significant enhancement of cytotoxic effect against MCF-7 breast cancer cell lines by photothermal therapy (PTT).
本报告的目的是推进纳米技术与近红外（NIR）激光介导治疗相结合，以提高治疗乳腺癌（MCF-7）细胞的效率。通过选择独特的分析方法，分析了表面物理化学相互作用和性能得到改善的纳米氧化石墨烯（nGO）片与硫化铜（CuS）量子点的相互作用。通过各种体外检测方法研究了 nGO 的细胞毒性作用。表面活化的 nGO 在 233nm 处表现出最大吸收峰。XRD 分析表明，石墨的强度降低，新峰出现在 2Ѳ=11.4o 左右，层间距为 0.728 至 0.828nm。此外，显微镜研究表明，合成的 nGO 呈现出透明层状，结构酥脆，并且有几层呈现出纳米薄片的形态。载有 CuSQDs 的 nGO 片的浓度对 MCF-7 乳腺癌细胞的细胞毒性增强有很大影响，评估的 IC50 值为 100μg/mL。荧光显微镜可视化证实了细胞凋亡形态学变化和 CuSQDs-nGO 处理的乳腺癌（MCF-7）细胞系中的细胞死亡。此外，在 CuSQDs-nGO 处理后，癌细胞 Caspase-3、ROS 和 LDH 活性的水平显著升高。因此，本详细的生物学研究表明，NIR 与表面活化的 CuSQDs-nGO 联合应用于光热疗法（PTT）对 MCF-7 乳腺癌细胞系具有显著的增强细胞毒性作用。
