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等离子体生成的一氧化氮水通过细胞内硝化应激介导环境传播的致病性细菌失活。

Plasma-Generated Nitric Oxide Water Mediates Environmentally Transmitted Pathogenic Bacterial Inactivation via Intracellular Nitrosative Stress.

机构信息

Plasma Bioscience Research Center, Department of Electrical and Biological Physics, Kwangwoon University, Seoul 01897, Republic of Korea.

Department of Biotechnology, College of Engineering, The University of Suwon, Hwaseong 18323, Republic of Korea.

出版信息

Int J Mol Sci. 2023 Jan 18;24(3):1901. doi: 10.3390/ijms24031901.

DOI:10.3390/ijms24031901
PMID:36768225
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9915551/
Abstract

Over time, the proportion of resistant bacteria will increase. This is a major concern. Therefore, effective and biocompatible therapeutic strategies against these bacteria are urgently needed. Non-thermal plasma has been exhaustively characterized for its antibacterial activity. This study aims to investigate the inactivation efficiency and mechanisms of plasma-generated nitric oxide water (PG-NOW) on pathogenic water, air, soil, and foodborne Gram-negative and Gram-positive bacteria. Using a colony-forming unit assay, we found that PG-NOW treatment effectively inhibited the growth of bacteria. Moreover, the intracellular nitric oxide (NO) accumulation was evaluated by 4-amino-5-methylamino-2',7'-dichlorofluorescein diacetate (DAF-FM DA) staining. The reduction of viable cells unambiguously indicates the anti-microbial effect of PG-NOW. The and genes are associated with nitrosative stress, and regulation corresponds to oxidative stress in bacterial cells. To support the nitrosative effect mediated by PG-NOW, we have further assessed the and gene expressions after treatment. Accordingly, expression was enhanced, whereas the expression was decreased following PG-NOW treatment. The disruption of cell morphology was observed using scanning electron microscopy (SEM) analysis. In conclusion, our findings furnish evidence of an initiation point for the further progress and development of PG-NOW-based antibacterial treatments.

摘要

随着时间的推移，耐药菌的比例将会增加。这是一个主要的关注点。因此，迫切需要针对这些细菌的有效且生物相容的治疗策略。非热等离子体的抗菌活性已经得到了广泛的研究。本研究旨在研究等离子体生成的亚硝态氮水（PG-NOW）对致病性水、空气、土壤和食源性病原体革兰氏阴性和革兰氏阳性细菌的灭活效率和机制。通过集落形成单位测定，我们发现 PG-NOW 处理能有效抑制细菌的生长。此外，通过 4-氨基-5-甲基氨基-2',7'-二氯荧光素二乙酸酯（DAF-FM DA）染色评估细胞内一氧化氮（NO）的积累。活细胞数量的减少明确表明了 PG-NOW 的抗菌作用。 和 基因与硝化应激有关， 调节对应于细菌细胞中的氧化应激。为了支持 PG-NOW 介导的硝化作用，我们进一步评估了处理后的 和 基因表达。结果表明，PG-NOW 处理后 表达增强， 表达减少。扫描电子显微镜（SEM）分析观察到细胞形态的破坏。总之，我们的研究结果为基于 PG-NOW 的抗菌治疗的进一步进展和发展提供了证据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17c5/9915551/58bf290d2b4d/ijms-24-01901-g009.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17c5/9915551/58bf290d2b4d/ijms-24-01901-g009.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17c5/9915551/fe448c09a682/ijms-24-01901-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17c5/9915551/1a350eef8f90/ijms-24-01901-g007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17c5/9915551/58bf290d2b4d/ijms-24-01901-g009.jpg

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