Wang Jing-Jing, Chen Qi, Li Yue-Zhong
State Key Laboratory of Microbial Technology, Institute of Microbiology Technology, Shandong University, Qingdao 266237, PR China.
Int J Syst Evol Microbiol. 2018 Nov;68(11):3518-3522. doi: 10.1099/ijsem.0.003022. Epub 2018 Sep 17.
A pale yellow bacterial strain, designated SDU1-6, was isolated from a soil sample collected in the Jinan campus of Shandong University, Shandong Province, PR China. The strain was aerobic, motile, Gram-stain-negative and rod-shaped. Cellular growth occurred at 13-37 °C, pH 5.0-10.0 and with 0-0.1 % (w/v) NaCl, and the optimal growth was at 25 °C and pH 7.5. SDU1-6 had the highest 16S rRNA gene similarity of 95.42 % with Chryseolinea serpens DSM 24574. The genome was 6 542 746 bp in length with 43.5 mol% DNA G+C content. The major fatty acids of SDU1-6 were C16 : 1ω5 and iso-C15 : 0, the major menaquinone was menaquinone-7 (MK-7) and the major polar lipid was phosphatidylethanolamine. On the basis of the polyphasic evidence and the 16S rRNA gene sequence, SDU1-6 represents a novel species of the genus Chryseolinea, for which the name Chryseolineaflava sp. nov. is proposed. The type strain is SDU1-6 (=CGMCC 1.13492=JCM 32520).
从中国山东省山东大学济南校区采集的土壤样本中分离出一株淡黄色细菌菌株,命名为SDU1-6。该菌株为需氧菌,具运动性,革兰氏染色阴性,呈杆状。细胞在13-37 °C、pH 5.0-10.0和0-0.1 %(w/v)NaCl条件下生长,最适生长温度为25 °C,最适pH为7.5。SDU1-6与蛇形金黄杆菌DSM 24574的16S rRNA基因相似度最高,为95.42 %。基因组长度为6 542 746 bp,DNA G+C含量为43.5 mol%。SDU1-6的主要脂肪酸为C16 : 1ω5和异-C15 : 0,主要甲基萘醌为甲基萘醌-7(MK-7),主要极性脂为磷脂酰乙醇胺。基于多相证据和16S rRNA基因序列,SDU1-6代表金黄杆菌属的一个新物种,为此提出金黄金黄杆菌(Chryseolineaflava sp. nov.)这一名称。模式菌株为SDU1-6(=CGMCC 1.13492=JCM 32520)。