School of Food Science and Biotechnology, Kyungpook National University, Daegu, 41566, Republic of Korea.
Department of Environmental Health Sciences, Graduate School of Public Health, Seoul National University, Seoul, 08826, Republic of Korea.
J Microbiol. 2019 Feb;57(2):170-179. doi: 10.1007/s12275-019-8610-0. Epub 2019 Jan 31.
Since Salmonella Enteritidis is one of the major foodborne pathogens, on-site applicable rapid detection methods have been required for its control. The purpose of this study was to isolate and purify S. Enteritidis-specific phage (KFS-SE2 phage) from an eel farm and to investigate its feasibility as a novel, efficient, and reliable bio-receptor for its employment. KFS-SE2 phage was successfully isolated at a high concentration of (2.31 ± 0.43) × 10 PFU/ml, and consisted of an icosahedral head of 65.44 ± 10.08 nm with a non-contractile tail of 135.21 ± 12.41 nm. The morphological and phylogenetic analysis confirmed that it belongs to the Pis4avirus genus in the family of Siphoviridae. KFS-SE2 genome consisted of 48,608 bp with 45.7% of GC content. Genome analysis represented KFS-SE2 to have distinctive characteristics as a novel phage. Comparative analysis of KFS-SE2 phage with closely related strains confirmed its novelty by the presence of unique proteins. KFS-SE2 phage exhibited excellent specificity to S. Enteritidis and was stable under the temperature range of 4 to 50°C and pH of 3 to 11 (P < 0.05). The latent time was determined to be 20 min. Overall, a new lytic KFS-SE2 phage was successfully isolated from the environment at a high concentration and the excellent feasibility of KFS-SE2 phage was demonstrated as a new bio-receptor for S. Enteritidis detection.
由于肠炎沙门氏菌是主要的食源性病原体之一，因此需要现场应用快速检测方法来进行控制。本研究旨在从鳗鱼养殖场中分离和纯化肠炎沙门氏菌特异性噬菌体（KFS-SE2 噬菌体），并研究其作为一种新型、高效、可靠的生物受体用于检测肠炎沙门氏菌的可行性。KFS-SE2 噬菌体在高浓度（2.31±0.43）×10PFU/ml 下成功分离，由一个 65.44±10.08nm 的二十面体头部和一个 135.21±12.41nm 的非收缩尾部组成。形态学和系统发育分析证实，它属于科的 Pis4avirus 属，Siphoviridae 科。KFS-SE2 基因组由 48608bp 组成，GC 含量为 45.7%。基因组分析表明，KFS-SE2 噬菌体具有独特的特征，是一种新型噬菌体。与密切相关菌株的 KFS-SE2 噬菌体比较分析证实，其独特蛋白的存在证明了其新颖性。KFS-SE2 噬菌体对肠炎沙门氏菌表现出优异的特异性，在 4 至 50°C 的温度范围和 3 至 11 的 pH 值下稳定（P<0.05）。潜伏期为 20 分钟。总之，从环境中以高浓度成功分离出一种新的裂解 KFS-SE2 噬菌体，并且证明 KFS-SE2 噬菌体作为肠炎沙门氏菌检测的新型生物受体具有优异的可行性。
