Graduate School of Nanobioscience, Yokohama City University, Kanazawa-ku, Yokohama 236-0027, Japan.
Graduate School of Nanobioscience, Yokohama City University, Kanazawa-ku, Yokohama 236-0027, Japan; Ichiban Life Corporation, Naka-ku, Yokohama 231-0048, Japan.
J Integr Med. 2019 Mar;17(2):141-146. doi: 10.1016/j.joim.2019.01.004. Epub 2019 Jan 12.
Keratinocytes are the predominant cell type in the epidermis and play key roles in epidermal function. Thus, identification of the compounds that regulate the growth of keratinocytes is of importance. Here we searched for such compounds from the herbs used in traditional medicine Ayurveda.
Human keratinocytes were cultured in the presence or absence of the herbal extracts for 2 weeks; the effect of the extracts on cell growth was determined by staining the cells with Coomassie brilliant blue. To detect the compounds that regulate the growth of keratinocytes, the herbal extracts were subjected to high-performance liquid chromatography (HPLC).
We found that the extract of Emblica officinalis enhanced the growth of keratinocytes in culture. Further, we fractionated the extract of E. officinalis using HPLC and identified the fractions responsible for the enhanced growth of keratinocytes.
The extract of E. officinalis enhanced the growth of human keratinocytes in culture. E. officinalis contains the compounds that would be beneficial for human skin health because enhanced growth of keratinocytes would promote wound healing.
角朊细胞是表皮中的主要细胞类型，在表皮功能中起关键作用。因此，鉴定调节角朊细胞生长的化合物非常重要。在这里，我们从传统医学阿育吠陀中使用的草药中寻找此类化合物。
将人角质形成细胞在存在或不存在草药提取物的情况下培养 2 周；用考马斯亮蓝染色细胞来确定提取物对角质形成细胞生长的影响。为了检测调节角质形成细胞生长的化合物，对草药提取物进行高效液相色谱 (HPLC) 分析。
我们发现余甘子的提取物可促进角质形成细胞在培养中的生长。此外，我们使用 HPLC 对余甘子的提取物进行了分级分离，并鉴定出了负责增强角质形成细胞生长的级分。
余甘子的提取物可促进人角质形成细胞在培养中的生长。余甘子含有对人类皮肤健康有益的化合物，因为增强角质形成细胞的生长会促进伤口愈合。
