Indian Institute of Integrative Medicine, Council of Scientific and Industrial Research, Canal Road, Jammu Tawi 180001, Jammu and Kashmir India.
J Ethnopharmacol. 2010 Oct 28;132(1):109-14. doi: 10.1016/j.jep.2010.07.047. Epub 2010 Aug 3.
Emblica officinalis fruit (EO), commonly known as Amla is a reputed traditional medicine and functional food used in Indian subcontinent. It has long been used in Indian folk medicine to treat liver diseases, stomach ulcers, inflammatory diseases, metabolic disorders, geriatric complaints, skin disorders and beauty care.
Recently, it has been shown to promote pro-collagen content and inhibit matrix metalloproteinase levels in skin fibroblast. The aim of the present study was to investigate the efficacy of EO to inhibit UVB-induced photo-aging in human skin fibroblasts.
Mitochondrial activity of human skin fibroblasts was measured by MTT-assay. Quantifications of pro-collagen 1 and matrix metalloproteinase 1 (MMP-1) release were performed by immunoassay techniques. Hyaluronidase inhibition assay was studied in vitro using bovine testicular hyaluronidase and human umbilical cord hyaluronic acid. Cell cycle analysis was performed by flowcytometry using propidium iodide.
EO stimulated, the otherwise UVB inhibited cellular proliferation and protected pro-collagen 1 against UVB-induced depletion via inhibition of UVB-induced MMP-1 in skin fibroblasts (10-40 μg/mL, p>0.001). EO exhibited inhibitory activity of hyaluronidase (10-40 μg/mL, p>0.001). Treatment with EO also prevented UVB disturbed cell cycle to normal phase.
The results of the present study suggests that EO effectively inhibits UVB-induced photo-aging in human skin fibroblast via its strong ROS scavenging ability and its therapeutic and cosmetic applications remain to be explored.
余甘子果（EO），俗称印度醋栗，是一种著名的传统药物和功能性食品，在印度次大陆被广泛使用。它在印度民间医学中被长期用于治疗肝脏疾病、胃溃疡、炎症性疾病、代谢紊乱、老年病、皮肤疾病和美容护理。
最近的研究表明，它可以促进皮肤成纤维细胞中前胶原的含量并抑制基质金属蛋白酶的水平。本研究的目的是研究 EO 抑制人皮肤成纤维细胞中 UVB 诱导的光老化的功效。
通过 MTT 测定法测量人皮肤成纤维细胞的线粒体活性。通过免疫测定技术定量测定前胶原 1 和基质金属蛋白酶 1（MMP-1）的释放。体外使用牛睾丸透明质酸酶和人脐带透明质酸研究透明质酸酶抑制试验。通过碘化丙啶进行流式细胞术进行细胞周期分析。
EO 刺激了皮肤成纤维细胞的增殖，否则 UVB 会抑制其增殖，并通过抑制 UVB 诱导的 MMP-1 来保护前胶原 1 免受 UVB 诱导的耗竭（10-40μg/mL，p>0.001）。EO 对透明质酸酶表现出抑制活性（10-40μg/mL，p>0.001）。用 EO 处理还可以防止 UVB 扰乱细胞周期至正常阶段。
本研究的结果表明，EO 通过其强大的 ROS 清除能力有效抑制人皮肤成纤维细胞中 UVB 诱导的光老化，其治疗和美容应用有待进一步探索。
