Mohamad Jamaludin, Masrudin Siti Saleha, Alias Zazali, Muhamad Nur Airina
Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Human Anatomy Department, Faculty of Medicine and Health Sciences, University Putra Malaysia, 43300, Selangor, Malaysia.
Mol Biol Rep. 2019 Apr;46(2):1855-1871. doi: 10.1007/s11033-019-04638-5. Epub 2019 Feb 1.
Pueraria mirifica (PM) is a medicinal plant native to Thailand contained high amount of phytoestrogen and possesses anticancer activity. This study reports the effect of P. mirifica extract, phytoestrogen of diadzein and genistein for its benign prostate hyperplasia properties in testosterone-induced prostate hyperplasia in male Sprague Dawley rats. The P. mirifica extract was evaluated for its total phenols, flavonoid and antioxidant activity using DPPH, FRAP and metal chelating assay. The assessment of P. mirifica, diadzein and genistein against benign prostate hyperplasia was determined in testosterone-induced prostate hyperplasia in male Sprague Dawley rats. The total phenol was higher than flavonoid but showed low antioxidant activity of DPPH, FRAP and metal chelating. The aqueous PM extract at 1000 mg/kg significantly increased testosterone levels in testosterone-induced rats by 13% while diadzein and genistein increased it by 11% and 17% respectively. However, levels of FSH, LH, triglyceride and HDL are not affected by the oral administration of PM, diadzein and genistein to the rats. Similarly, total protein, albumin, globulin, total bilirubin, conjugated bilirubin, alkaline phosphatase, alanine aminotransferase, AST, and G-glutamyltransferase showed no significant difference as compared with negative control rats. The body weight of the rats, testis, kidney and liver showed no toxic effect. The zinc content increased significantly and the zinc transporter gen of ZnT4 and ZIP4 highly expressed suggesting that the PM, diadzein and genistein plays essential role in modulating prostate zinc homeostasis. Similarly, the expression of IL-6, AR and ER was significantly reduced indicating functioning in regulation of prostate growth and acts as anti-inflammatory role in preventing BPH. In conclusion, the results indicated that PM reduced BPH and contributed to the regulation in the zinc transport expression of the prostate cells in the benign prostate hyperplasia (BPH).
野葛根是一种原产于泰国的药用植物，含有大量植物雌激素，并具有抗癌活性。本研究报道了野葛根提取物、大豆苷元和染料木黄酮等植物雌激素对雄性斯普拉格-道利大鼠睾酮诱导的前列腺增生的良性前列腺增生特性的影响。使用DPPH、FRAP和金属螯合测定法评估了野葛根提取物的总酚、黄酮类化合物和抗氧化活性。在雄性斯普拉格-道利大鼠睾酮诱导的前列腺增生中，测定了野葛根、大豆苷元和染料木黄酮对良性前列腺增生的作用。总酚含量高于黄酮类化合物，但DPPH、FRAP和金属螯合的抗氧化活性较低。1000mg/kg的野葛根水提取物使睾酮诱导的大鼠睾酮水平显著升高13%，而大豆苷元和染料木黄酮分别使其升高11%和17%。然而，大鼠口服野葛根、大豆苷元和染料木黄酮后，促卵泡激素、促黄体生成素、甘油三酯和高密度脂蛋白水平不受影响。同样，与阴性对照大鼠相比，总蛋白、白蛋白、球蛋白、总胆红素、结合胆红素、碱性磷酸酶、丙氨酸转氨酶、天冬氨酸转氨酶和γ-谷氨酰转移酶无显著差异。大鼠的体重、睾丸、肾脏和肝脏均未显示出毒性作用。锌含量显著增加，锌转运体基因ZnT4和ZIP4高度表达，表明野葛根、大豆苷元和染料木黄酮在调节前列腺锌稳态中起重要作用。同样，白细胞介素-6、雄激素受体和雌激素受体的表达显著降低，表明其在调节前列腺生长中发挥作用，并在预防良性前列腺增生中起抗炎作用。总之，结果表明野葛根可减轻良性前列腺增生，并有助于调节良性前列腺增生（BPH）中前列腺细胞的锌转运表达。
