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定量洞察蓝藻细胞经济。

Quantitative insights into the cyanobacterial cell economy.

机构信息

Laboratory of Adaptive Biotechnologies, Global Change Research Institute CAS, Brno, Czech Republic.

Institut für Biologie, Fachinstitut für Theoretische Biologie, Humboldt-Universität zu Berlin, Berlin, Germany.

出版信息

Elife. 2019 Feb 4;8:e42508. doi: 10.7554/eLife.42508.

DOI:10.7554/eLife.42508
PMID:30714903
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6391073/
Abstract

Phototrophic microorganisms are promising resources for green biotechnology. Compared to heterotrophic microorganisms, however, the cellular economy of phototrophic growth is still insufficiently understood. We provide a quantitative analysis of light-limited, light-saturated, and light-inhibited growth of the cyanobacterium sp. PCC 6803 using a reproducible cultivation setup. We report key physiological parameters, including growth rate, cell size, and photosynthetic activity over a wide range of light intensities. Intracellular proteins were quantified to monitor proteome allocation as a function of growth rate. Among other physiological acclimations, we identify an upregulation of the translational machinery and downregulation of light harvesting components with increasing light intensity and growth rate. The resulting growth laws are discussed in the context of a coarse-grained model of phototrophic growth and available data obtained by a comprehensive literature search. Our insights into quantitative aspects of cyanobacterial acclimations to different growth rates have implications to understand and optimize photosynthetic productivity.

摘要

光养微生物是绿色生物技术有前途的资源。然而，与异养微生物相比，光养生长的细胞经济仍未得到充分理解。我们使用可重复的培养装置对蓝藻 sp. PCC 6803 的光限制、光饱和和光抑制生长进行了定量分析。我们报告了一系列关键的生理参数，包括在广泛的光强范围内的生长速率、细胞大小和光合作用活性。为了监测生长速率作为功能的蛋白质组分配，我们对细胞内蛋白质进行了定量。除了其他生理适应之外，我们还发现随着光强和生长速率的增加，翻译机制上调，而光捕获组件下调。在光养生长的粗粒度模型和通过全面文献搜索获得的可用数据的背景下，讨论了由此产生的生长规律。我们对蓝藻适应不同生长速率的定量方面的见解对于理解和优化光合作用生产力具有重要意义。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b273/6391073/69c6c2403710/elife-42508-fig6-figsupp2.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b273/6391073/8c96e7a291a0/elife-42508-fig6-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b273/6391073/69c6c2403710/elife-42508-fig6-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b273/6391073/cd642972e368/elife-42508-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b273/6391073/3ee4a8567727/elife-42508-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b273/6391073/3ec70be00462/elife-42508-fig2-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b273/6391073/2bebdf381c6b/elife-42508-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b273/6391073/273786e62f57/elife-42508-fig3-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b273/6391073/6d220c6f0677/elife-42508-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b273/6391073/f185d283e1fc/elife-42508-fig4-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b273/6391073/d629e7fdbf44/elife-42508-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b273/6391073/5efd3dbe4467/elife-42508-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b273/6391073/8c96e7a291a0/elife-42508-fig6-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b273/6391073/69c6c2403710/elife-42508-fig6-figsupp2.jpg

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