IRHS (Institut de Recherche en Horticulture et Semences), UMR 1345, INRA, Agrocampus-Ouest, Université d'Angers, QuaSaV, Beaucouzé, France.
Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, Versailles, France.
J Exp Bot. 2019 Feb 20;70(4):1349-1365. doi: 10.1093/jxb/ery454.
Small secreted peptides are important players in plant development and stress response. Using a targeted in silico approach, we identified a family of 14 Arabidopsis genes encoding precursors of serine-rich endogenous peptides (PROSCOOP). Transcriptomic analyses revealed that one member of this family, PROSCOOP12, is involved in processes linked to biotic and oxidative stress as well as root growth. Plants defective in this gene were less susceptible to Erwinia amylovora infection and showed an enhanced root growth phenotype. In PROSCOOP12 we identified a conserved motif potentially coding for a small secreted peptide. Exogenous application of synthetic SCOOP12 peptide induces various defense responses in Arabidopsis. Our findings show that SCOOP12 has numerous properties of phytocytokines, activates the phospholipid signaling pathway, regulates reactive oxygen species response, and is perceived in a BAK1 co-receptor-dependent manner.
小分泌肽在植物发育和应激反应中起着重要作用。我们采用靶向的计算机方法，鉴定出拟南芥编码富含丝氨酸的内源性肽前体（PROSCOOP）的 14 个基因家族。转录组分析显示，该家族的一个成员 PROSCOOP12 参与了与生物和氧化应激以及根系生长相关的过程。该基因缺失的植物对梨火疫病菌的感染敏感性降低，并且表现出增强的根生长表型。在 PROSCOOP12 中，我们鉴定出一个保守的基序，可能编码一个小分泌肽。外源性应用合成的 SCOOP12 肽可诱导拟南芥产生各种防御反应。我们的研究结果表明，SCOOP12 具有多种植物细胞因子的特性，激活磷脂信号通路，调节活性氧反应，并以 BAK1 共受体依赖性方式被感知。
