MARE-Marine and Environmental Sciences Centre, ESTM, Instituto Politécnico de Leiria, 2520-641 Peniche, Portugal.
Department of Pharmacology, Faculty of Veterinary, University of Santiago de Compostela, 27002 Lugo, Spain.
Mar Drugs. 2019 Feb 1;17(2):85. doi: 10.3390/md17020085.
is a marine brown seaweed mainly found on the Atlantic coast. Herein, we report the antioxidant and neuroprotective activities of seven fractions (F1⁻F7) obtained by normal phase chromatography from the dichloromethane extract, as well as of its two major isolated diterpenes. Total phenolic content of fractions was determined by the Folin⁻Ciocalteu method, while antioxidant activity was evaluated by the DPPH, ORAC, and FRAP assays. Neuroprotective effects were evaluated in a neurotoxic model induced by 6-hydroxydopamine (6-OHDA) in a human neuroblastoma cell line (SH-SY5Y), while the mechanisms associated to neuroprotection were investigated by the determination of mitochondrial membrane potential, H₂O₂ production, Caspase-3 activity, and by observation of DNA fragmentation. Fractions F4 and F5 exhibited the best neuroprotective and antioxidant activities, respectively. F4 fraction prevented changes in mitochondrial potential, and induced a reduction of H₂O₂ levels production and an increase in cell viability, suggesting that it may contain multi-target compounds acting on different pathways. Hence, this fraction was subjected to purification steps, affording the known diterpenes eleganolone and eleganonal. Both compounds exhibited antioxidant potential, being interesting candidates for further neuroprotective studies.
是一种主要生长在大西洋沿岸的海洋褐藻。在此，我们报告了从二氯甲烷提取物中通过正相色谱法获得的七个馏分（F1⁻F7）及其两种主要分离出的二萜的抗氧化和神经保护活性。通过福林酚比色法测定馏分的总酚含量，通过 DPPH、ORAC 和 FRAP 测定评估抗氧化活性。通过 6-羟多巴胺（6-OHDA）在人神经母细胞瘤细胞系（SH-SY5Y）中诱导的神经毒性模型评估神经保护作用，同时通过测定线粒体膜电位、H₂O₂产生、Caspase-3 活性和观察 DNA 片段化来研究与神经保护相关的机制。馏分 F4 和 F5 分别表现出最好的神经保护和抗氧化活性。F4 馏分可防止线粒体电位变化，并诱导 H₂O₂ 水平产生减少和细胞活力增加，表明它可能含有作用于不同途径的多靶化合物。因此，该馏分进行了纯化步骤，得到了已知的二萜 eleganolone 和 eleganonal。这两种化合物都表现出抗氧化潜力，是进一步神经保护研究的有前途的候选物。
