超临界二氧化碳萃取叶片中的精油：抗氧化和抗菌活性

Essential Oil Extracted from Leaves by Supercritical Carbon Dioxide: Antioxidant and Antimicrobial Activities.

作者信息

Wu Hong, Li Jilie, Jia Yuan, Xiao Zhihong, Li Peiwang, Xie Yixian, Zhang Aihua, Liu Rukuan, Ren Zewen, Zhao Mengrui, Zeng Chaozhen, Li Changzhu

机构信息

Key Laboratory of Cultivation and Protection Co-constructed for Non-wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha 410004, China.

Key Laboratory of State Forestry Administration on Utilization Science for Southern Woody Oil Resource, Hunan Academy of Forestry, Changsha 410004, China.

出版信息

J Anal Methods Chem. 2019 Jan 2;2019:8192439. doi: 10.1155/2019/8192439. eCollection 2019.

DOI:10.1155/2019/8192439

PMID:30719374

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6334621/

Abstract

To improve essential oil quality, especially to reserve the thermal instability of compounds, supercritical CO extraction (SFE) was applied to recover essential oil from leaves. A response surface methodology was applied to optimize the extraction process. The highest essential oil yield was predicted at extraction time 120 min, extraction pressure 25 MPa, extraction temperature 35°C, and CO flow 18 L/h for the SFE processing. Under these experimental conditions, the mean essential oil yield is 4.40%. In addition, the chemical compositions of SFE were compared with those obtained by hydrodistillation extraction (HD). There were 41 compounds obtained of SFE, while 35 compounds of HD. Alcohols and aldehydes were the main compositions in the essential oils. Furthermore, the antioxidant activities and antimicrobial of essential oils obtained by HD and the evaluated condition of SFE were compared. Results showed that the antioxidant activities of SFE oil are better than those of HD. Minimum inhibitory concentrations (MICs) were determined by the microdilution method. Essential oil obtained from SFE and HD exhibited a significant antimicrobial activity against all tested microorganisms. It is confirmed that the SFE method can be an alternative processing method to extract essential oils from leaves.

摘要

为提高精油品质，尤其是保留化合物的热不稳定性，采用超临界CO₂萃取（SFE）从叶片中提取精油。应用响应面法优化萃取工艺。对于SFE工艺，预测在萃取时间120分钟、萃取压力25MPa、萃取温度35°C和CO₂流速18L/h时精油产率最高。在这些实验条件下，平均精油产率为4.40%。此外，将SFE所得的化学成分与水蒸馏萃取（HD）所得的进行了比较。SFE得到41种化合物，而HD得到35种化合物。醇类和醛类是精油中的主要成分。此外，还比较了HD所得精油以及SFE评估条件下精油的抗氧化活性和抗菌性能。结果表明，SFE精油的抗氧化活性优于HD精油。通过微量稀释法测定最低抑菌浓度（MIC）。SFE和HD所得精油对所有测试微生物均表现出显著的抗菌活性。证实SFE方法可作为从叶片中提取精油的替代加工方法。