Abdel-Farid I B, Sheded M G, Mohamed E A
Botany Department, Faculty of Science, Aswan University, Aswan 81528, Egypt ; Biology Department, Faculty of Science, Aljouf University, Sakaka, Saudi Arabia.
Botany Department, Faculty of Science, Aswan University, Aswan 81528, Egypt.
Saudi J Biol Sci. 2014 Nov;21(5):400-8. doi: 10.1016/j.sjbs.2014.03.005. Epub 2014 Mar 31.
Metabolomic profiling of different parts (leaves, flowers and pods) of Acacia species (Acacia nilotica, Acacia seyal and Acacia laeta) was evaluated. The multivariate data analyses such as principal component analysis (PCA) and partial least square-discriminant analysis (PLS-DA) were used to differentiate the distribution of plant metabolites among different species or different organs of the same species. A. nilotica was characterized with a high content of saponins and A. seyal was characterized with high contents of proteins, phenolics, flavonoids and anthocyanins. A. laeta had a higher content of carbohydrates than A. nilotica and A. seyal. On the basis of these results, total antioxidant capacity, DPPH free radical scavenging activity and reducing power of the methanolic extracts of studied parts were evaluated. A. nilotica and A. seyal extracts showed less inhibitory concentration 50 (IC50) compared to A. laeta extracts which means that these two species have the strongest radical scavenging activity whereas A. laeta extracts have the lowest radical scavenging activity. A positive correlation between saponins and flavonoids with total antioxidant capacity and DPPH radical scavenging activity was observed. Based on these results, the potentiality of these plants as antioxidants was discussed.
对阿拉伯胶树属物种（阿拉伯胶树、塞内加尔阿拉伯胶树和莱氏阿拉伯胶树）不同部位（叶片、花朵和豆荚）进行了代谢组学分析。采用主成分分析（PCA）和偏最小二乘判别分析（PLS - DA）等多变量数据分析方法，以区分不同物种之间或同一物种不同器官之间植物代谢物的分布情况。阿拉伯胶树的特征是皂苷含量高，塞内加尔阿拉伯胶树的特征是蛋白质、酚类、黄酮类和花青素含量高。莱氏阿拉伯胶树的碳水化合物含量高于阿拉伯胶树和塞内加尔阿拉伯胶树。基于这些结果，对所研究部位的甲醇提取物的总抗氧化能力、DPPH自由基清除活性和还原能力进行了评估。与莱氏阿拉伯胶树提取物相比，阿拉伯胶树和塞内加尔阿拉伯胶树提取物的半数抑制浓度（IC50）较低，这意味着这两个物种具有最强的自由基清除活性，而莱氏阿拉伯胶树提取物的自由基清除活性最低。观察到皂苷和黄酮类与总抗氧化能力和DPPH自由基清除活性之间存在正相关。基于这些结果，讨论了这些植物作为抗氧化剂的潜力。
