Choi Jiwon, Yang Dahye, Chhetri Geeta, Cha Seho, Seo Taegun
Department of Life Science, Dongguk University-Seoul, Goyang, 10326, South Korea.
Antonie Van Leeuwenhoek. 2019 Jul;112(7):1047-1054. doi: 10.1007/s10482-019-01238-y. Epub 2019 Feb 6.
Strain U15, rod-shaped, catalase and oxidase positive, non-motile, hot pink pigmented, Gram-negative bacterium, was isolated from soil of Udo port, Udo Island, South Korea. Growth was observed at 10-48 °C, pH 6-11, and 0% (w/v) NaCl. Optimum growth conditions are 30-40 °C, pH 7-10, and 0% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA sequences showed that strain U15 forms a distinct clade with type strains of the family Cytophagaceae, with similarities below 89%. The major polar lipids are phosphatidylethanolamine and phosphatidylserine. Strain U15 was found to contain MK-7 as the only menaquinone, and iso-C, Cω5c, iso-C 3-OH and summed feature 3 (comprising Cω7c and/or Cω6c) as the major fatty acids (> 10%). The DNA G + C content of strain U15 was determined to be 54.3 mol%. Based on the phylogenetic, phenotypic characteristics and chemotaxonomic analysis data, strain U15 (= KCTC 62116 = JCM 32361) should be classified as representing a novel species of a novel genus within the family Cytophagaceae for which the name Tellurirhabdus rosea gen. nov., sp. nov., is proposed.
菌株U15为杆状，过氧化氢酶和氧化酶呈阳性，无运动性，呈热粉色色素，革兰氏阴性菌，从韩国济州岛牛岛港口的土壤中分离得到。在10 - 48°C、pH值6 - 11和0%（w/v）NaCl条件下可观察到其生长。最适生长条件为30 - 40°C、pH值7 - 10和0%（w/v）NaCl。基于16S rRNA序列的系统发育分析表明，菌株U15与噬纤维菌科的模式菌株形成一个独特的分支，相似度低于89%。主要极性脂类为磷脂酰乙醇胺和磷脂酰丝氨酸。发现菌株U15仅含有MK - 7作为唯一的甲基萘醌，主要脂肪酸（>10%）为异C、Cω5c、异C 3 - OH和总和特征3（包含Cω7c和/或Cω6c）。菌株U15的DNA G + C含量测定为54.3 mol%。基于系统发育、表型特征和化学分类分析数据，菌株U15（= KCTC 62116 = JCM 32361）应被分类为代表噬纤维菌科内一个新属的新物种，为此提出新属名Tellurirhabdus rosea gen. nov., sp. nov.。
