Arshi Asghar, Hosseini Sayed Mostafa, Hosseini Fataneh Saleh Khaje, Amiri Zahra Yousefnejad, Hosseini Fatemeh Sadat, Sheikholia Lavasani Mahsa, Kerdarian Hossein, Dehkordi Maryam Safarpour
Human Genetic Research Center, Baqiyatallah University of Medical Science, Tehran, Iran.
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Mol Biol Rep. 2019 Apr;46(2):2059-2066. doi: 10.1007/s11033-019-04656-3. Epub 2019 Feb 5.
Derived from rosaceous plant seed, amygdalin belongs to aromatic cyanogenic glycoside group, and its anticancer effects have been supported by mounting evidence. In this study, we objected to investigate amygdalin effect on two antiapoptotic genes (Survivin, XIAP) and two lncRNAs (GAS5, MALAT1) in human cancer cells (A549, MCF7, AGS). Employing RT-qPCR analysis, we compared the mRNA levels of the genes related to apoptosis in A549, MCF7, and AGS cancer cells between amygdalin-treated (24, 48 and 72 h) and un-treated groups. RNA was extracted from both cell groups and then cDNAs were synthesized. The changes in the gene expression levels were specified using ΔΔCt method. RT-qPCR analysis has revealed that the expression of Survivin, XIAP, GAS5 and MALAT1 in amygdala-treated cancer cells were significantly different, compared to the un-treated cells. However, these expressions were different depending on the treatment time. According to the results, amygdalin significantly inhibited the expression level of Survivin, and XIAP genes in treated via untreated group. Our findings suggest that amygdalin might have an anticancer effect due to the various gene expressions in A549, MCF7, and AGS human cancer cells, showing it's potential as a natural therapeutic anticancer drug.
苦杏仁苷来源于蔷薇科植物种子，属于芳香族氰苷类，其抗癌作用已得到越来越多证据的支持。在本研究中，我们旨在研究苦杏仁苷对人癌细胞（A549、MCF7、AGS）中两个抗凋亡基因（Survivin、XIAP）和两个长链非编码RNA（GAS5、MALAT1）的影响。采用RT-qPCR分析，我们比较了苦杏仁苷处理组（24、48和72小时）和未处理组的A549、MCF7和AGS癌细胞中与凋亡相关基因的mRNA水平。从两组细胞中提取RNA，然后合成cDNA。使用ΔΔCt法确定基因表达水平的变化。RT-qPCR分析显示，与未处理的细胞相比，苦杏仁苷处理的癌细胞中Survivin、XIAP、GAS5和MALAT1的表达存在显著差异。然而，这些表达因处理时间而异。根据结果，苦杏仁苷在处理组中显著抑制了Survivin和XIAP基因的表达水平，与未处理组相比。我们的研究结果表明，苦杏仁苷可能由于A549、MCF7和AGS人癌细胞中的各种基因表达而具有抗癌作用，显示出其作为天然治疗性抗癌药物的潜力。
