School of Chemical Science and Engineering, Tongji University, Shanghai, 200092, China.
State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai, 200092, China.
Sci Rep. 2019 Feb 7;9(1):1624. doi: 10.1038/s41598-018-36609-4.
There has been a developing technology in algae with pharmaceuticals wastewater. However, the effect and the underlying mechanism of pharmaceuticals on algae are not well understood. To investigate the effect and mechanism of pharmaceuticalson microalgae, four pharmaceuticals of clofibric acid (CLF), ciprofloxacin (CIP), diclofenac (DCF) and carbamazepine (CBZ) on C. pyrenoidosa culture were analyzed. At low concentrations (<10 mg/L), the pharmaceuticals, especially the DCF, exhibited positive effects on both the structure and function of algal cultures; algal growth (i.e., chlorophyll a accumulation, lipid accumulation) and activities of antioxidant enzymes were stimulated. The algal metabolite differences of various DCF concentrations were investigated and a total of 91 substances were identified, whose samples were clustered and clearly separated. The key metabolomics pathway analysis found that the DCF promoted the carbohydrate and fatty acid metabolic pathway in C. pyrenoidosa under relatively low concentrations (<10 mg/L). However, the algae metabolomics pathway was disturbed significantly under the action of a high concentration of DCF (>100 mg/L). The study detected the effects of four pharmaceuticals on C. pyrenoidosa and demonstrated that the usage of metabolomics analysis complemented with DCF could be an effective approach to understand the mechanism of molecular evolution in C. pyrenoidosa for microalgal biomass and bioenergy from wastewater in researches of biological resources.
藻类处理制药废水中的技术不断发展。然而，制药对藻类的影响及其潜在机制尚不清楚。为了研究制药对微藻的影响和机制，分析了 4 种药物：氯苯酸（CLF）、环丙沙星（CIP）、双氯芬酸（DCF）和卡马西平（CBZ）对 C. pyrenoidosa 培养的影响。在低浓度（<10mg/L）下，这些药物，特别是 DCF，对藻类培养的结构和功能均表现出积极影响；刺激藻类生长（即叶绿素 a 积累、脂质积累）和抗氧化酶活性。研究了不同 DCF 浓度下藻类代谢物的差异，共鉴定出 91 种物质，其样本聚类且明显分离。关键代谢组学途径分析发现，在相对较低浓度（<10mg/L）下，DCF 促进了 C. pyrenoidosa 中的碳水化合物和脂肪酸代谢途径。然而，在高浓度 DCF（>100mg/L）的作用下，藻类代谢组学途径受到显著干扰。该研究检测了 4 种药物对 C. pyrenoidosa 的影响，表明代谢组学分析与 DCF 结合使用可能是理解 C. pyrenoidosa 分子进化机制的有效方法，有助于从废水生物资源中获取微藻生物质和生物能源的研究。
