Debnath Priyanka, Mondal Arghadip, Hajra Amita, Das Chittaranjan, Mondal Naba Kumar
Environmental Chemistry Laboratory, Department of Environmental Science, The University of Burdwan, West Bengal, India.
J Genet Eng Biotechnol. 2018 Dec;16(2):519-526. doi: 10.1016/j.jgeb.2018.07.007. Epub 2018 Aug 3.
The present study evaluates the cytogenetic effects of both silver and gold nanoparticles on the root cells of . In this study, the root cells of were treated with both gold and silver nanoparticles of different concentrations (1 mg/L, 5 mg/L and 10 mg/L) along with control for 72 h. Experimental results revealed that after 72 h of exposure, a significant decrease in mitotic index (MI) from 68% (control) to 52.4% (1 mg/L), 47.3% (5 mg/L) and 41.4% (10 mg/L) for gold nanoparticles and 57.1% (1 mg/L), 53% (5 mg/l), 55.8% (10 mg/L) for silver nanoparticles. Through minute observation of the photograph, it was recorded that some specific chromosomal abnormalities such as stickiness of chromosome, chromosome breaks, nuclear notch, and clumped chromosome at different exposure conditions. Therefore, present results clearly suggest that root tip assay could be a viable path through which negative impact of both gold and silver nanoparticles can be demonstrated over a wide range of concentrations.
本研究评估了银纳米颗粒和金纳米颗粒对[植物名称]根细胞的细胞遗传学效应。在本研究中，[植物名称]的根细胞用不同浓度（1毫克/升、5毫克/升和10毫克/升）的金纳米颗粒和银纳米颗粒处理，并设置对照组，处理72小时。实验结果显示，暴露72小时后，金纳米颗粒处理组的有丝分裂指数（MI）从对照组的68%显著下降至52.4%（1毫克/升）、47.3%（5毫克/升）和41.4%（10毫克/升）；银纳米颗粒处理组的有丝分裂指数分别为57.1%（1毫克/升）、53%（5毫克/升）、55.8%（10毫克/升）。通过对照片的仔细观察，记录到在不同暴露条件下出现了一些特定的染色体异常，如染色体粘连、染色体断裂、核凹陷和染色体聚集。因此，目前的结果清楚地表明，[植物名称]根尖试验可能是一条可行的途径，通过它可以在广泛的浓度范围内证明金纳米颗粒和银纳米颗粒两者的负面影响。
