Volga Region State Academy of Physical Culture, Sports and Tourism, Kazan, Russia.
Kazan Federal University, Kazan, Russia.
Biomed Res Int. 2019 Jan 10;2019:9843781. doi: 10.1155/2019/9843781. eCollection 2019.
The aim of this study was to assess changes in skin microbiota of wrestlers during training sessions and to determine the sensitivity of hemolytic bacterial isolates to antiseptics.
The main skin bacterial isolates obtained from the skin of 15 wrestlers were identified by cultivation method, with the following MALDI Biotyper and 16S rRNA gene sequencing methods. The sensitivity of hemolytic isolates to antiseptics (Veltosept-2, Cutasept F, Chlorhexidine, Miramistin, and Hydrogen Peroxide) was evaluated by measuring the size of bacterial growth inhibition zone on agar plates.
Opportunistic bacteria of the species , , and were the most commonly found species in skin microbiota of wrestlers before and after training sessions. Representatives of all these species mostly had a hemolytic activity. An alcohol-containing antiseptic Veltosept-2 showed the strongest inhibitory effect on the bacterial isolates of athletes' skin microbiota most frequently detected in this study.
The general increase in the bacterial colonization of wrestlers' skin, as well as the presence of hemolytic forms of opportunistic bacteria in cutaneous microbiota, indicates dysbiotic changes and a decrease in the protective features of the host organism. Veltosept-2 application can reduce the incidence of skin infections in contact sports athletes with the highest efficiency.
本研究旨在评估摔跤运动员在训练过程中皮肤微生物群的变化，并确定溶血性细菌分离物对防腐剂的敏感性。
从 15 名摔跤运动员的皮肤中获得主要皮肤细菌分离物，通过培养法进行鉴定，并采用 MALDI Biotyper 和 16S rRNA 基因测序方法进行鉴定。通过测量琼脂平板上细菌生长抑制区的大小来评估溶血性分离物对防腐剂（Veltosept-2、Cutasept F、氯己定、Miramistin 和过氧化氢）的敏感性。
在训练前后，运动员皮肤微生物群中最常见的机会性病原体为 、 、 和 。这些物种的代表都具有很强的溶血活性。含酒精的防腐剂 Veltosept-2 对本研究中最常检测到的运动员皮肤微生物群中的细菌分离物表现出最强的抑制作用。
摔跤运动员皮肤细菌定植的普遍增加，以及皮肤微生物群中存在机会性溶血性细菌，表明微生态失调变化和宿主生物保护特性的下降。Veltosept-2 的应用可以以最高的效率降低接触运动运动员皮肤感染的发生率。
