应用田口方法优化细菌藻酸盐 - 氧化铜生物纳米复合材料的抗癌活性。
Applying the Taguchi Method to the Optimization of Anticancer Activity of Bacterial Alginate-CuO Bionanocomposite.
作者信息
Golshah Amin, Taran Mojtaba, Safaei Mohsen, Mozaffari Hamid Reza, Imani Mohammad Moslem, Sharifi Roohollah, Moradpoor Hedaiat, Upadhyay Prabhat
机构信息
Department of Orthodontics, School of Dentistry, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Department of Nanobiotechnology, Faculty of Science, Razi University, Kermanshah, Iran.
出版信息
Open Access Maced J Med Sci. 2018 Dec 20;7(1):1-5. doi: 10.3889/oamjms.2019.006. eCollection 2019 Jan 15.
AIM
In recent decades, despite various types of cancer inflicting many people worldwide, the existing therapies are not satisfactory and have many side effects. The present study was conducted to optimise the synthesis of novel alginate-CuO nanocomposite with utmost anticancer activity.
METHODS
In this study, 9 nanocomposites were designed using Taguchi method and three factors including copper oxide nanoparticles, alginate biopolymer and stirring times were assessed at three different levels. The anticancer activity of the synthesised nanocomposites was evaluated on the MCF-7 cell line using the MTT method. Using the Qulitek-4 software, we determined the optimum conditions for the synthesis of alginate-CuO nanocomposite with the highest anticancer activity.
RESULTS
The results indicated that all three factors (copper oxide, alginate and stirring time) were effective on the anticancer activity of the alginate-CuO nanocomposite. Also, the nanocomposite produced under the conditions of experiment 9 (8 mg/ml of copper oxide, 2 mg/ml of alginate and 60 min of stirring time) provided the highest growth inhibition rate as 75.63% against cancer cells.
CONCLUSION
The synthesised alginate-copper oxide nanocomposites in this study showed a significant anticancer effect. Therefore, the synthesised nanocomposite under optimal conditions can be used in the design of new anticancer drugs.
目的
近几十年来，尽管全球有多种癌症折磨着众多人群，但现有的治疗方法并不令人满意且有许多副作用。本研究旨在优化具有最大抗癌活性的新型藻酸盐 - 氧化铜纳米复合材料的合成。
方法
在本研究中，使用田口方法设计了9种纳米复合材料，并在三个不同水平下评估了包括氧化铜纳米颗粒、藻酸盐生物聚合物和搅拌时间在内的三个因素。使用MTT法在MCF - 7细胞系上评估合成的纳米复合材料的抗癌活性。使用Qulitek - 4软件，我们确定了具有最高抗癌活性的藻酸盐 - 氧化铜纳米复合材料合成的最佳条件。
结果
结果表明，所有三个因素（氧化铜、藻酸盐和搅拌时间）对藻酸盐 - 氧化铜纳米复合材料的抗癌活性均有影响。此外，在实验9的条件下制备的纳米复合材料（8 mg/ml的氧化铜、2 mg/ml的藻酸盐和60分钟的搅拌时间）对癌细胞的生长抑制率最高，为75.63%。
结论
本研究中合成的藻酸盐 - 氧化铜纳米复合材料显示出显著的抗癌效果。因此，在最佳条件下合成的纳米复合材料可用于新型抗癌药物的设计。
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