Department of Biotechnology, Kongunadu Arts and Science College, Coimbatore 029, Tamilnadu, India.
Department of Biotechnology, Kongunadu Arts and Science College, Coimbatore 029, Tamilnadu, India.
Int J Biol Macromol. 2019 Dec 1;141:476-483. doi: 10.1016/j.ijbiomac.2019.08.235. Epub 2019 Aug 29.
Polymer functionalized metal oxide nanocomposites are great interest due to wide range of application, especially in nanomedicine. The present study reports an eco-friendly bio-inspired synthesis of chitosan/copper oxide (CS-CuO) nanocomposite for the first time using rutin. The bio-synthesized CS-CuO nanocomposite was characterized using UV-Visible spectroscopy, FE-SEM, EDS, TEM, XRD and FTIR analyses. FE-SEM and TEM images revealed the synthesized CS-CuO nanocomposite having spherical shaped structure with an average size of 10-30 nm. EDS analysis confirmed the elements present in synthesized CS-CuO nanocomposite. FTIR studies revealed the role of rutin and chitosan for reduction, capping and synthesis of CS-CuO nanocomposite from the precursor copper salt. The XRD analysis revealed monoclinic structure of CS-CuO nanocomposite. Anti-proliferative activity of the CS-CuO nanocomposite was evaluated in human lung cancer cell line A549. Synthesized CS-CuO nanocomposite showed concentration-depended anti-proliferative activity against A549 cancer cells and their IC value was found to be 20 ± 0.50 μg/mL. Furthermore, synthesized nanocomposite induce apoptosis in treated A549 cancer cells assayed by AO/EtBr fluorescent staining method. In conclusion, the synthesized CS-CuO nanocomposite using rutin can be used as a potential anticancer agent in biomedical and clinical sectors.
聚合物功能化金属氧化物纳米复合材料因其广泛的应用而备受关注，尤其是在纳米医学领域。本研究首次报道了一种使用芦丁的环保仿生合成壳聚糖/氧化铜（CS-CuO）纳米复合材料的方法。采用 UV-Visible 光谱、FE-SEM、EDS、TEM、XRD 和 FTIR 分析对生物合成的 CS-CuO 纳米复合材料进行了表征。FE-SEM 和 TEM 图像显示，合成的 CS-CuO 纳米复合材料具有球形结构，平均尺寸为 10-30nm。EDS 分析证实了合成的 CS-CuO 纳米复合材料中存在的元素。FTIR 研究表明，芦丁和壳聚糖在 CS-CuO 纳米复合材料的还原、封端和合成中起作用，其前体为铜盐。XRD 分析表明 CS-CuO 纳米复合材料具有单斜结构。采用 AO/EtBr 荧光染色法评价了 CS-CuO 纳米复合材料对人肺癌细胞系 A549 的抗增殖活性。合成的 CS-CuO 纳米复合材料对 A549 癌细胞表现出浓度依赖性的抗增殖活性，其 IC 值为 20±0.50μg/mL。此外，通过 AO/EtBr 荧光染色法检测到合成的纳米复合材料在处理的 A549 癌细胞中诱导凋亡。总之，使用芦丁合成的 CS-CuO 纳米复合材料可以作为生物医学和临床领域的潜在抗癌药物。
