Zou Zhi, Zhang Xicai
Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture and Rural Affairs, Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, P. R. China.
Danzhou Investigation & Experiment Station of Tropical Crops, Ministry of Agriculture and Rural Affairs, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, P. R. China.
PeerJ. 2019 Feb 5;7:e6354. doi: 10.7717/peerj.6354. eCollection 2019.
DNA-binding with one finger (Dof) proteins comprise a plant-specific transcription factor family involved in plant growth, development and stress responses. This study presents a genome-wide comparison of Dof family genes in physic nut () and castor bean (), two Euphorbiaceae plants that have not experienced any recent whole-genome duplication. A total of 25 or 24 Dof genes were identified from physic nut and castor genomes, respectively, where JcDof genes are distributed across nine out of 11 chromosomes. Phylogenetic analysis assigned these genes into nine groups representing four subfamilies, and 24 orthologous groups were also proposed based on comparison of physic nut, castor, and rice Dofs. Conserved microsynteny was observed between physic nut and castor Dof-coding scaffolds, which allowed anchoring of 23 RcDof genes to nine physic nut chromosomes. In contrast to how no recent duplicate was present in castor, two tandem duplications and one gene loss were found in the Dof gene family of physic nut. Global transcriptome profiling revealed diverse patterns of Jc/RcDof genes over various tissues, and key Dof genes involved in flower development and stress response were also identified in physic nut. These findings provide valuable information for further studies of Dof genes in physic nut and castor.
单指DNA结合（Dof）蛋白构成了一个植物特有的转录因子家族，参与植物生长、发育及胁迫响应。本研究对麻风树（）和蓖麻（）这两种大戟科植物中的Dof家族基因进行了全基因组比较，这两种植物近期未经历过全基因组复制。分别从麻风树和蓖麻基因组中鉴定出了25个或24个Dof基因，其中麻风树Dof基因分布在11条染色体中的9条上。系统发育分析将这些基因分为代表四个亚家族的九个组，并且基于麻风树、蓖麻和水稻Dof的比较还提出了24个直系同源组。在麻风树和蓖麻的Dof编码支架之间观察到保守的微同线性，这使得23个蓖麻Dof基因能够定位到九条麻风树染色体上。与蓖麻中不存在近期重复基因不同，在麻风树的Dof基因家族中发现了两次串联重复和一次基因丢失。全转录组分析揭示了麻风树/蓖麻Dof基因在不同组织中的多样表达模式，并且在麻风树中还鉴定出了参与花发育和胁迫响应的关键Dof基因。这些发现为进一步研究麻风树和蓖麻中的Dof基因提供了有价值的信息。
