Key Laboratory of Tropical Plant Resource and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming 650223, China.
Faculty of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.
Int J Mol Sci. 2018 May 29;19(6):1598. doi: 10.3390/ijms19061598.
Physic nut ( L.) is a species of flowering plant with great potential for biofuel production and as an emerging model organism for functional genomic analysis, particularly in the Euphorbiaceae family. DNA binding with one finger (Dof) transcription factors play critical roles in numerous biological processes in plants. Nevertheless, the knowledge about members, and the evolutionary and functional characteristics of the gene family in physic nut is insufficient. Therefore, we performed a genome-wide screening and characterization of the gene family within the physic nut draft genome. In total, 24 genes (encoding 33 JcDof proteins) were identified. All the genes were divided into three major groups based on phylogenetic inference, which was further validated by the subsequent gene structure and motif analysis. Genome comparison revealed that segmental duplication may have played crucial roles in the expansion of the gene family, and gene expansion was mainly subjected to positive selection. The expression profile demonstrated the broad involvement of genes in response to various abiotic stresses, hormonal treatments and functional divergence. This study provides valuable information for better understanding the evolution of genes, and lays a foundation for future functional exploration of genes.
麻疯树是一种具有巨大生物燃料生产潜力的开花植物，也是功能基因组分析的新兴模式生物，特别是在大戟科家族中。具有一个手指 DNA 结合（Dof）的转录因子在植物的许多生物学过程中起着关键作用。然而，关于麻疯树中的成员以及该基因家族的进化和功能特征的知识还不够充分。因此，我们在麻疯树的草案基因组中进行了全基因组筛选和特征分析。总共鉴定出 24 个基因（编码 33 个 JcDof 蛋白）。所有基因都根据系统发育推断分为三大类，随后的基因结构和基序分析进一步验证了这一点。基因组比较表明，片段复制可能在基因家族的扩张中发挥了关键作用，基因的扩张主要受到正选择的影响。表达谱表明，基因广泛参与了对各种非生物胁迫、激素处理和功能分化的反应。本研究为更好地理解基因的进化提供了有价值的信息，并为未来基因的功能探索奠定了基础。
