Herbal Medicine Research Center, Institute for Medical Research, Ministry of Health Malaysia, 50588, Kuala Lumpur, Malaysia.
Infectious Disease Research Center, Institute for Medical Research, Ministry of Health Malaysia, 50588, Kuala Lumpur, Malaysia.
BMC Complement Altern Med. 2019 Feb 11;19(1):44. doi: 10.1186/s12906-019-2438-3.
Carica papaya leaves have been used for traditional treatment of dengue fever and have been reported to exhibit an immunomodulatory activity by affecting the level of cytokine production in vitro and in vivo. Due to the lack of adequate in vivo evidence in dengue disease model, the present study was initiated to screen and identify the cytokines affected by freeze-dried C. papaya leaf juice (FCPLJ) treatment in AG129 mice infected with DEN-2 dengue virus.
The AG129 mice were fed orally with FCPLJ for 3 consecutive days after 24 h of dengue virus inoculation. Plasma cytokines were screened by using ProcartaPlex immunoassay. The gene expression in the liver was analyzed by using RT Profiler PCR Array.
The results showed that FCPLJ treatment has increased the plasma CCL2/MCP-1 level during peak of viremia. Gene expression study has identified 8 inflammatory cytokine genes which were downregulated in the liver of infected AG129 mice treated with FCPLJ. The downregulated inflammatory cytokine genes were CCL6/MRP-1, CCL8/MCP-2, CCL12/MCP-5, CCL17/TARC, IL1R1, IL1RN/IL1Ra, NAMPT/PBEF1 and PF4/CXCL4.
The findings indicated the possible immunomodulatory role of FCPLJ during dengue virus infection in AG129 mice.
木瓜叶被用于传统治疗登革热，并被报道具有免疫调节活性，可通过影响细胞因子的产生水平来实现，无论是在体外还是体内。由于缺乏登革热疾病模型中的充分体内证据，本研究旨在筛选和鉴定受冻干木瓜叶汁（FCPLJ）处理影响的细胞因子，该处理作用于感染 DEN-2 登革热病毒的 AG129 小鼠。
在感染登革热病毒 24 小时后，AG129 小鼠连续 3 天口服 FCPLJ。通过 ProcartaPlex 免疫分析筛选血浆细胞因子。通过 RT Profiler PCR 阵列分析肝脏中的基因表达。
结果表明，FCPLJ 处理可在病毒血症高峰期增加血浆 CCL2/MCP-1 水平。基因表达研究表明，FCPLJ 处理可下调感染 AG129 小鼠肝脏中 8 种炎症细胞因子基因的表达。下调的炎症细胞因子基因包括 CCL6/MRP-1、CCL8/MCP-2、CCL12/MCP-5、CCL17/TARC、IL1R1、IL1RN/IL1Ra、NAMPT/PBEF1 和 PF4/CXCL4。
研究结果表明，FCPLJ 在 AG129 小鼠感染登革热病毒期间可能具有免疫调节作用。
