Taychaworaditsakul Weerakit, Saenjum Chalermpong, Lumjuan Nongkran, Chawansuntati Kriangkrai, Sawong Suphunwadee, Jaijoy Kanjana, Na Takuathung Mingkwan, Sireeratawong Seewaboon
Clinical Research Center for Food and Herbal Product Trials and Development (CR-FAH), Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
Department of Pharmacology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
Toxics. 2024 Mar 1;12(3):198. doi: 10.3390/toxics12030198.
leaves, traditionally utilized in dietary supplements and pharmaceuticals, exhibit a broad spectrum of potentially therapeutic properties, including anti-inflammatory, antimalarial, and wound healing properties. This study examined the acute and chronic toxicity of 10% ethanolic-extracted leaf in Sprague Dawley rats. The acute toxicity assessment was a single oral dose of 5000 mg/kg body weight, while the chronic toxicity assessment included daily oral doses of 100, 400, 1000, and 5000 mg/kg over 180 days. Systematic monitoring covered a range of physiological and behavioral parameters, including body and organ weights. End-point evaluations encompassed hematological and biochemical analyses, along with gross and histopathological examinations of internal organs. Findings revealed no acute toxicity in the leaf extract group, although a significant decrease in uterine weight was observed without accompanying histopathology abnormalities. In the chronic toxicity assessment, no statistically significant differences between the control and the leaf extract groups were detected across multiple measures, including behavioral, physiological, and hematological indices. Importantly, histopathological examination corroborated the absence of any tissue abnormalities. The study results indicate that leaf extract exhibited no adverse effects on the rats during the 180-day oral administration period, affirming its potential safety for prolonged usage.
树叶，传统上用于膳食补充剂和药品，具有广泛的潜在治疗特性，包括抗炎、抗疟疾和伤口愈合特性。本研究检测了10%乙醇提取的树叶对斯普拉格-道利大鼠的急性和慢性毒性。急性毒性评估为单次口服剂量5000毫克/千克体重，而慢性毒性评估包括在180天内每日口服剂量100、400、1000和5000毫克/千克。系统监测涵盖一系列生理和行为参数，包括体重和器官重量。终点评估包括血液学和生化分析，以及内部器官的大体和组织病理学检查。结果显示树叶提取物组未出现急性毒性，尽管观察到子宫重量显著下降，但无组织病理学异常。在慢性毒性评估中，在包括行为、生理和血液学指标在内的多项测量中，对照组和树叶提取物组之间未检测到统计学上的显著差异。重要的是，组织病理学检查证实没有任何组织异常。研究结果表明，在180天的口服给药期内，树叶提取物对大鼠没有不良影响，证实了其长期使用的潜在安全性。
