Kumar Thankanadar Arul, Veeravarmal Veeran, Nirmal Ramdas Madhavan, Amsaveni Ramamoorthy, Nassar Mohamed Hanifa Mohamed, Kesavan Ganesan
Department of Oral and Maxillofacial Pathology, Rajah Muthiah Dental College and Hospital, Annamalai University, Chidambaram, Tamil Nadu, India.
Department of Oral Pathology, Madha Dental College and Hospital, Chennai, Tamil Nadu, India.
Indian J Dermatol. 2019 Jan-Feb;64(1):41-46. doi: 10.4103/ijd.IJD_350_16.
BACKGROUND/PURPOSE: Lichen planus is a T-cell-mediated mucocutaneous disorder characterized histopathologically by a band of chronic inflammatory cells in the subepithelial zone and degeneration of basal layer. The present study was aimed to evaluate the distribution and quantitative assessment of cluster of differentiation 1a (CD1a)-positive Langerhans cells (LCs) in oral lichen planus (OLP), thus to determine the role of LCs pertaining to the changes occurring in OLP.
Five cases of normal oral mucosa and 20 cases of OLP were immunostained with CD1a antibody; the positive cells were counted manually in the photomicrographs and statistically analyzed using -test, Mann-Whitney test, and Wilcoxon signed-rank test.
The average percentage of CD1a-positive LCs in normal subjects was 0.9%, and in the OLP cases higher percentage was observed (3.93%). The statistical comparison of these two parameters was significant (=0.018). The degree of basal cell degeneration and density of subepithelial infiltrate on statistical comparison with the concentration of CD1a-positive LCs showed significant results.
LCs play a pivotal role in the recruitment of CD4+ and CD8+ cells to the subepithelial region and basal keratinocytes apoptosis. A small number of study subjects, assessment of only CD1a molecule and LCs in the epidermis only were a few of the drawbacks of the study.
背景/目的：扁平苔藓是一种T细胞介导的黏膜皮肤疾病，其组织病理学特征为上皮下区有一条慢性炎症细胞带以及基底层变性。本研究旨在评估口腔扁平苔藓（OLP）中分化簇1a（CD1a）阳性朗格汉斯细胞（LCs）的分布及定量评估，从而确定LCs在OLP发生变化中的作用。
用CD1a抗体对5例正常口腔黏膜和20例OLP进行免疫染色；在显微照片中手动计数阳性细胞，并使用t检验、曼-惠特尼检验和威尔科克森符号秩检验进行统计分析。
正常受试者中CD1a阳性LCs的平均百分比为0.9%，而在OLP病例中观察到更高的百分比（3.93%）。这两个参数的统计比较具有显著性（P=0.018）。与CD1a阳性LCs浓度进行统计比较时，基底细胞变性程度和上皮下浸润密度显示出显著结果。
LCs在CD4+和CD8+细胞募集到上皮下区域以及基底角质形成细胞凋亡中起关键作用。研究对象数量少、仅评估表皮中的CD1a分子和LCs是该研究的一些不足之处。
