Charpentier Emilie, Doudet Ludovic, Allart-Simon Ingrid, Colin Marius, Gangloff Sophie C, Gérard Stéphane, Reffuveille Fany
EA 4691 Biomatériaux et Inflammation en Site Osseux (BIOS), UFR Pharmacie, Université de Reims Champagne-Ardenne, SFR Cap Santé (FED 4231), 51097 Reims, France.
Institut de Chimie Moléculaire de Reims (ICMR-UMR CNRS 7312), UFR Pharmacie, Université de Reims Champagne-Ardenne, 51097 Reims, France.
Antibiotics (Basel). 2021 Oct 4;10(10):1205. doi: 10.3390/antibiotics10101205.
Antibiotic treatments can participate in the formation of bacterial biofilm in case of under dosage. The interest of indoloquinoline scaffold for drug discovery incited us to study the preparation of new indolo [2,3-]quinoline derivatives by a domino radical process. We tested the effect of two different "indoloquinoline" molecules (Indol-1 and Indol-2) without antimicrobial activity, in addition to ciprofloxacin, on biofilm formation thanks to crystal violet staining and enumeration of adhered bacteria. This association of ciprofloxacin and Indol-1 or Indol-2 attenuated the formation of biofilm up to almost 80% compared to ciprofloxacin alone, or even prevented the presence of adhered bacteria. In conclusion, these data prove that the association of non-antimicrobial molecules with an antibiotic can be a solution to fight against biofilm and antibiotic resistance emergence.
抗生素治疗在剂量不足的情况下可能会促使细菌生物膜的形成。吲哚喹啉骨架在药物研发方面的价值促使我们研究通过多米诺自由基过程制备新型吲哚[2,3 -]喹啉衍生物。除环丙沙星外，我们还测试了两种无抗菌活性的不同“吲哚喹啉”分子（吲哚 - 1和吲哚 - 2）对生物膜形成的影响，通过结晶紫染色和对黏附细菌的计数来评估。与单独使用环丙沙星相比，环丙沙星与吲哚 - 1或吲哚 - 2联合使用可将生物膜的形成减弱近80%，甚至可防止黏附细菌的存在。总之，这些数据证明非抗菌分子与抗生素联合使用可能是对抗生物膜和抗生素耐药性产生的一种解决方案。
