Hormones Department, National Research Centre, Giza, Egypt.
Stem Cells Lab, Center of Excellence for Advanced Sciences, National Research Centre, Giza, Egypt.
J Mater Sci Mater Med. 2019 Feb 12;30(2):24. doi: 10.1007/s10856-019-6224-z.
The main target of this work was to explore the proliferative impact of selenium dioxide nanoparticles (SeO) and selenium dioxide/titanium dioxide nanocomposites (Se/Ti (I), (II) and (III)) on mesenchymal stem cells (MSCs). For this purpose, SeO and Se/Ti (I), (II) and (III) were prepared by facile one step method and characterized by transmission electron microscopy (TEM), Zetasizer, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM) along with energy-dispersive X-ray spectrometry (EDX) with reference to SeO nanoparticles. Also, MSCs were isolated from rat bone marrow (BM-MSCs) and adipose tissue (ADSCs), propagated and characterized by flow cytometry. Thereafter, the proliferative effect of the fabricated nanomaterials was investigated by MTT assay. The TEM and DLS results, revealed that the average particle size of the suggested nanomaterials was in nanoscale. XRD pattern showed well crystalline structure for SeO nanoparticles and Se/Ti (I), (II) and (III) nanocomposites; the decreasing of the crystalline phase was observed by increasing the wt% of TiO. The designed nanomaterials showed proliferative effects on MSCs with the most prominent effect exerted by 2 µg/ml of Se/Ti (III) and 5 µg/ml of Se/Ti (II) for ADSCs and 20 µg/ml of Se/Ti (II) and 10 µg/ml of Se/Ti (III) for BM-MSCs. Therefore, these newly designed nanomaterials have a promising influence on MSCs proliferation and they are recommended to be utilized in the filed of tissue engineering.
这项工作的主要目的是探索二氧化硒纳米粒子（SeO）和二氧化硒/二氧化钛纳米复合材料（Se/Ti（I）、（II）和（III））对间充质干细胞（MSCs）的增殖影响。为此，采用简便的一步法制备了 SeO 和 Se/Ti（I）、（II）和（III），并通过透射电子显微镜（TEM）、Zetasizer、X 射线衍射（XRD）、傅里叶变换红外光谱（FT-IR）和扫描电子显微镜（SEM）以及能量色散 X 射线光谱（EDX）对 SeO 纳米粒子进行了表征。此外，从大鼠骨髓（BM-MSCs）和脂肪组织（ADSCs）中分离出 MSCs，通过流式细胞术进行了增殖和表征。然后，通过 MTT 测定法研究了所制备的纳米材料的增殖效果。TEM 和 DLS 结果表明，所提出的纳米材料的平均粒径在纳米范围内。XRD 图谱表明，SeO 纳米粒子和 Se/Ti（I）、（II）和（III）纳米复合材料具有良好的结晶结构；随着 TiO 重量百分比的增加，观察到结晶相减少。所设计的纳米材料对 MSCs 表现出增殖作用，其中最显著的作用是 2µg/ml 的 Se/Ti（III）和 5µg/ml 的 Se/Ti（II）对 ADSCs，以及 20µg/ml 的 Se/Ti（II）和 10µg/ml 的 Se/Ti（III）对 BM-MSCs。因此，这些新设计的纳米材料对 MSCs 的增殖有很大的影响，建议将它们应用于组织工程领域。
