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人滋养层细胞迁移和侵袭需要半乳糖凝集素-3。

Human trophoblast requires galectin-3 for cell migration and invasion.

机构信息

Laboratory for Biology of Reproduction, Institute for the Application of Nuclear Energy, University of Belgrade, Banatska 31b, 11080, Belgrade, Serbia.

King's College London, Faculty of Life Sciences & Medicine, Department of Women & Children's Health, Guy's Hospital, London SE1 9RT, London, United Kingdom.

出版信息

Sci Rep. 2019 Feb 14;9(1):2136. doi: 10.1038/s41598-018-38374-w.

DOI:10.1038/s41598-018-38374-w
PMID:30765738
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6376043/
Abstract

Invasive extravillous cytotrophoblast of the human placenta expresses galectins-1, -3, and -8 in vivo and in vitro. This study aimed to investigate the potential role of galectin-3 in cell migration and invasion, using recombinant human galectin-3 (rhgalectin-3), small molecule galectin inhibitor I, and galectin-3 silencing. HTR-8/SVneo cell migration was stimulated by rhgalectin-3 and reduced by I, which could be neutralised by rhgalectin-3. Inhibitor specificity and selectivity for the galectins expressed in extravillous trophoblast were validated in solid phase assays using recombinant galectin-1, -3, -8, confirming selectivity for galectin-3. HTR-8/SVneo cell migration and invasion, and invasion by isolated trophoblast cells in primary culture were significantly reduced in the presence of I which could be restored by rhgalectin-3. Upon HTR-8/SVneo cell treatment with galectin-3 siRNA both LGALS3 and galectin-3 protein were dramatically decreased. Silencing of galectin-3 induced significant reduction in cell migration and invasion, which was restored by rhgalectin-3. The influence on known mediators of cell invasion, MMP2 and -9, and integrins α, α, and β was followed in silenced cells, showing lower levels of MMPs and a large reduction in integrin subunit β. These results show that galectin-3 acts as a pro-invasive autocrine/paracrine factor in trophoblast in vitro.

摘要

人胎盘绒毛外滋养细胞中侵袭性的细胞滋养层表达半乳糖凝集素-1、-3 和-8 体内和体外。本研究旨在探讨半乳糖凝集素-3 在细胞迁移和侵袭中的潜在作用，使用重组人半乳糖凝集素-3（rhgalectin-3）、小分子半乳糖凝集素抑制剂 I 和半乳糖凝集素-3 沉默。rhgalectin-3 刺激 HTR-8/SVneo 细胞迁移，I 减少细胞迁移，rhgalectin-3 可中和 I 的作用。在使用重组半乳糖凝集素-1、-3、-8 的固相测定中验证了抑制剂对半乳糖凝集素在绒毛外滋养细胞中表达的特异性和选择性，证实了对半乳糖凝集素-3 的选择性。在存在 I 的情况下，HTR-8/SVneo 细胞迁移和侵袭以及分离的原代滋养细胞侵袭明显减少，rhgalectin-3 可恢复这种作用。在用 galectin-3 siRNA 处理 HTR-8/SVneo 细胞后，LGALS3 和半乳糖凝集素-3 蛋白均显著减少。沉默半乳糖凝集素-3 诱导细胞迁移和侵袭显著减少，rhgalectin-3 可恢复这种作用。随后研究了沉默细胞中已知的细胞侵袭介质 MMP2 和 MMP9 以及整合素α、α 和β 的影响，结果显示 MMPs 水平较低，整合素亚基β大量减少。这些结果表明，半乳糖凝集素-3 在体外滋养细胞中作为一种促侵袭的自分泌/旁分泌因子发挥作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e80/6376043/22a60b266d2f/41598_2018_38374_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e80/6376043/07a336abad6e/41598_2018_38374_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e80/6376043/144de9a796b4/41598_2018_38374_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e80/6376043/8b5df8b6179a/41598_2018_38374_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e80/6376043/ad350a82b5db/41598_2018_38374_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e80/6376043/eda2f404effe/41598_2018_38374_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e80/6376043/404dfe367df5/41598_2018_38374_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e80/6376043/22a60b266d2f/41598_2018_38374_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e80/6376043/07a336abad6e/41598_2018_38374_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e80/6376043/144de9a796b4/41598_2018_38374_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e80/6376043/8b5df8b6179a/41598_2018_38374_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e80/6376043/ad350a82b5db/41598_2018_38374_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e80/6376043/eda2f404effe/41598_2018_38374_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e80/6376043/404dfe367df5/41598_2018_38374_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e80/6376043/22a60b266d2f/41598_2018_38374_Fig7_HTML.jpg

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本文引用的文献

1
Galectins at a glance.半乳糖凝集素速览。
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2
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Invest Ophthalmol Vis Sci. 2018 Jan 1;59(1):223-230. doi: 10.1167/iovs.17-23196.
3
Galectin-3 Inhibition by a Small-Molecule Inhibitor Reduces Both Pathological Corneal Neovascularization and Fibrosis.小分子抑制剂对半乳糖凝集素-3的抑制作用可减轻病理性角膜新生血管化和纤维化。
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Int J Mol Sci. 2024 Sep 20;25(18):10096. doi: 10.3390/ijms251810096.
4
Bridging the Gap between Galectin-3 Expression and Hypertensive Pregnancy Disorders: A Narrative Review.连接半乳糖凝集素-3表达与高血压妊娠疾病之间的差距：一项叙述性综述。
J Clin Med. 2024 Aug 8;13(16):4636. doi: 10.3390/jcm13164636.
5
Fetal growth restriction induced by maternal gal-3 deficiency is associated with altered gut-placenta axis.母体半乳糖凝集素-3缺乏引起的胎儿生长受限与肠道-胎盘轴改变有关。
Cell Death Dis. 2024 Aug 8;15(8):575. doi: 10.1038/s41419-024-06962-6.
6
Spatial multiomics map of trophoblast development in early pregnancy.妊娠早期滋养层发育的空间多组学图谱。
Nature. 2023 Apr;616(7955):143-151. doi: 10.1038/s41586-023-05869-0. Epub 2023 Mar 29.
7
Roles of N-linked glycosylation and glycan-binding proteins in placentation: trophoblast infiltration, immunomodulation, angiogenesis, and pathophysiology.N 连接糖基化和糖结合蛋白在胎盘形成中的作用：滋养细胞浸润、免疫调节、血管生成和病理生理学。
Biochem Soc Trans. 2023 Apr 26;51(2):639-653. doi: 10.1042/BST20221406.
8
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9
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J Clin Med. 2022 Nov 3;11(21):6521. doi: 10.3390/jcm11216521.
Invest Ophthalmol Vis Sci. 2017 Jan 1;58(1):9-20. doi: 10.1167/iovs.16-20009.
4
Extracellular matrix in epitheliochorial, endotheliochorial and haemochorial placentation and its potential application for regenerative medicine.上皮绒毛膜、内皮绒毛膜和血绒毛膜胎盘形成中的细胞外基质及其在再生医学中的潜在应用。
Reprod Domest Anim. 2017 Feb;52(1):3-15. doi: 10.1111/rda.12868. Epub 2016 Dec 7.
5
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8
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9
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10
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