Departament de Ciència Animal, Universitat de Lleida-Agrotecnio Center, 25198, Lleida, Spain.
IRTA, Genètica i Millora Animal, 25198, Lleida, Spain.
Sci Rep. 2019 Feb 14;9(1):2031. doi: 10.1038/s41598-019-38622-7.
The adipogenic nature of the Iberian pig defines many quality attributes of its fresh meat and dry-cured products. The distinct varieties of Iberian pig exhibit great variability in the genetic parameters for fat deposition and composition in muscle. The aim of this work is to identify common and distinct genomic regions related to fatty acid composition in Retinto, Torbiscal, and Entrepelado Iberian varieties and their reciprocal crosses through a diallelic experiment. In this study, we performed GWAS using a high density SNP array on 382 pigs with the multimarker regression Bayes B method implemented in GenSel. A number of genomic regions showed strong associations with the percentage of saturated and unsaturated fatty acid in intramuscular fat. In particular, five regions with Bayes Factor >100 (SSC2 and SSC7) or >50 (SSC2 and SSC12) explained an important fraction of the genetic variance for miristic, palmitoleic, monounsaturated (>14%), oleic (>10%) and polyunsaturated (>5%) fatty acids. Six genes (RXRB, PSMB8, CHGA, ACACA, PLIN4, PLIN5) located in these regions have been investigated in relation to intramuscular composition variability in Iberian pigs, with two SNPs at the RXRB gene giving the most consistent results on oleic and monounsaturated fatty acid content.
伊比利亚猪的脂肪生成特性决定了其新鲜肉类和干式腌制产品的许多质量属性。不同品种的伊比利亚猪在肌肉中脂肪沉积和组成的遗传参数上表现出很大的可变性。本工作的目的是通过二倍体实验鉴定与雷蒂诺、托比斯卡和恩特雷佩拉多伊比利亚品种的脂肪酸组成相关的常见和独特基因组区域及其回交。在这项研究中，我们使用高密度 SNP 阵列在 382 头猪上进行了 GWAS，使用 GenSel 中实现的多标记回归贝叶斯 B 方法进行了 GWAS。许多基因组区域与肌肉内脂肪中饱和和不饱和脂肪酸的百分比表现出强烈的关联。特别是，五个 Bayes 因子>100（SSC2 和 SSC7）或>50（SSC2 和 SSC12）的区域解释了 miristic、棕榈油酸、单不饱和（>14%）、油酸（>10%）和多不饱和（>5%）脂肪酸遗传方差的重要部分。位于这些区域的六个基因（RXRB、PSMB8、CHGA、ACACA、PLIN4、PLIN5）已被研究与伊比利亚猪肌肉组成的变异性有关，RXRB 基因的两个 SNP 在油酸和单不饱和脂肪酸含量上给出了最一致的结果。
