Kaita Yasuhiko, Tarui Takehiko, Yoshino Hideaki, Matsuda Takeaki, Yamaguchi Yoshihiro, Nakagawa Takatoshi, Asahi Michio, Ii Masaaki
Department of Trauma and Critical Care Medicine, Kyorin University School of Medicine, Tokyo, Japan.
Division of Cardiology, Second Department of Internal Medicine, Kyorin University, School of Medicine, Tokyo, Japan.
Regen Ther. 2019 Feb 1;10:92-103. doi: 10.1016/j.reth.2019.01.001. eCollection 2019 Jun.
The purpose of this study was to evaluate whether cryopreserved (frozen) adipose-derived regenerative cells (ADRCs) have a therapeutic effect on burn wound healing as well as freshly isolated (fresh) ADRCs.
Full thickness burns were created on dorsum of nude mice and burn wound was excised. The wound was covered by artificial dermis with; (i) fresh ADRCs, (ii) frozen ADRCs, and (iii) PBS (control). The assessment for wound healing was performed by morphological, histopathological and immunohistochemical analyses.
In vivo analyses exhibited the significant therapeutic effect of frozen ADRCs on burn wound healing up to the similar or higher level of fresh ADRCs. There were significant differences of wound closure, epithelized tissue thickness, and neovascularization between the treatment groups and control group. Although there was no significant difference of therapeutic efficacy between fresh ADRC group and frozen ADRC group, frozen ADRCs improved burn wound healing process in dermal regeneration with increased great type I collagen synthesis compared with fresh ADRCs.
These findings indicate that frozen ADRCs allow us to apply not only quickly but also for multiple times, and the cryopreserved ADRCs could therefore be useful for the treatment of burn wounds in clinical settings.
本研究的目的是评估冷冻保存的（冻存的）脂肪来源的再生细胞（ADRCs）以及新鲜分离的（新鲜的）ADRCs对烧伤创面愈合是否具有治疗作用。
在裸鼠背部制造全层烧伤并切除烧伤创面。创面用人工真皮覆盖，分别为：（i）新鲜ADRCs，（ii）冻存ADRCs，以及（iii）PBS（对照）。通过形态学、组织病理学和免疫组织化学分析对创面愈合进行评估。
体内分析显示，冻存ADRCs对烧伤创面愈合具有显著治疗作用，达到与新鲜ADRCs相似或更高的水平。治疗组与对照组在伤口闭合、上皮化组织厚度和新血管形成方面存在显著差异。虽然新鲜ADRCs组和冻存ADRCs组之间的治疗效果没有显著差异，但与新鲜ADRCs相比，冻存ADRCs通过增加I型胶原蛋白的合成改善了烧伤创面愈合过程中的真皮再生。
这些发现表明，冻存ADRCs不仅能让我们快速应用，还能多次应用，因此冻存的ADRCs可能对临床治疗烧伤创面有用。
