Institute of Chemistry and The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem, 91904. Israel.
Nanoscale. 2019 May 9;11(18):8752-8759. doi: 10.1039/c8nr10043h.
The accumulation of bacteria on surfaces is currently one of the greatest concerns for the management of proper healthcare systems, water and energy. Here, we describe the mechanism by which a single peptide forms two pH-dependent supramolecular particles that resist bacterial contamination. By using NMR and molecular dynamics (MD), we determined the structures of the peptide monomers and showed the forces directing the self-assembly of each structure under different conditions. These peptide assemblies change the characteristics of bare glass and confer it with the ability to prevent biofilm formation. Furthermore, they can adsorb and release active compounds as demonstrated with an anticancer drug, antibiotic and enzyme. This synergism and the detailed understanding of the processes are necessary for developing new sterile surfaces for healthcare systems, water purification devices, food packaging or any environment that suffers from biocontamination.
目前，表面细菌的积累是妥善管理医疗保健系统、水和能源的最大关注点之一。在这里，我们描述了一种单一肽形成两种 pH 依赖性超分子颗粒的机制，这些颗粒能抵抗细菌污染。通过使用 NMR 和分子动力学（MD），我们确定了肽单体的结构，并展示了在不同条件下引导每种结构自组装的力。这些肽组装物改变了裸露玻璃的特性，并赋予其防止生物膜形成的能力。此外，正如用抗癌药物、抗生素和酶所证明的那样，它们可以吸附和释放活性化合物。这种协同作用和对过程的详细了解对于开发新的医疗保健系统、水净化装置、食品包装或任何遭受生物污染的环境的无菌表面是必要的。
