CSIRO Manufacturing, Research Way, Clayton, VIC, 3168, Australia.
Chimie ParisTech, Rue Pierre et Marie Curie, 75005, Paris, France.
Sci Rep. 2020 Sep 25;10(1):15796. doi: 10.1038/s41598-020-72478-6.
Inspired by the interesting natural antimicrobial properties of honey, biohybrid composite materials containing a low-fouling polymer hydrogel network and an encapsulated antimicrobial peroxide-producing enzyme have been developed. These synergistically combine both passive and active mechanisms for reducing microbial bacterial colonization. The mechanical properties of these materials were assessed using compressive mechanical analysis, which revealed these hydrogels possessed tunable mechanical properties with Young's moduli ranging from 5 to 500 kPa. The long-term enzymatic activities of these materials were also assessed over a 1-month period using colorimetric assays. Finally, the passive low-fouling properties and active antimicrobial activity against a leading opportunistic pathogen, Staphylococcus epidermidis, were confirmed using bacterial cell counting and bacterial adhesion assays. This study resulted in non-adhesive substrate-permeable antimicrobial materials, which could reduce the viability of planktonic bacteria by greater than 7 logs. It is envisaged these new biohybrid materials will be important for reducing bacterial adherence in a range of industrial applications.
受蜂蜜具有有趣的天然抗菌特性的启发，开发了含有低污染聚合物水凝胶网络和封装抗菌过氧化物产生酶的生物杂交复合材料。这些材料协同结合了被动和主动机制，以减少微生物细菌定植。使用压缩力学分析评估了这些材料的机械性能，结果表明这些水凝胶具有可调节的机械性能，杨氏模量范围为 5 至 500kPa。使用比色分析评估了这些材料在 1 个月期间的长期酶活性。最后，使用细菌细胞计数和细菌粘附实验证实了这些材料对主要机会性病原体表皮葡萄球菌的被动低污染特性和主动抗菌活性。这项研究产生了非粘附基质渗透性抗菌材料，可使浮游菌的存活率降低 7 个对数以上。预计这些新型生物杂交材料将在各种工业应用中减少细菌附着方面发挥重要作用。
