Piao Chunnan, Tian Mei, Gao Hongjun, Gao Yanxiao, Ruan Jianlei, Wu Lina, Gao Gang, Yi Lirong, Liu Jianxiang
Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing, China.
Department of Clinical Laboratory, Emergency General Hospital, Beijing, China.
Dose Response. 2020 Feb 3;18(1):1559325820902338. doi: 10.1177/1559325820902338. eCollection 2020 Jan-Mar.
To analyze changes in immune functions by detecting lymphocyte subsets in the peripheral blood of residents in the vicinity of radon from hot springs.
Two groups were randomly selected; 61 residents in the vicinity of the hot springs were assigned to the radon group, and 51 residents with a similar lifestyle and habits but no contact with hot springs were assigned to the control group. The percentages of lymphocyte subsets (CD3, CD4CD8, CD4CD8, CD4/CD8, and TCR/CD3) in the 2 groups were evaluated on a FACS Aria flow cytometer. The absolute values of lymphocytes (LYMPH#) and percentages of lymphocytes (LYMPH%) were measured by an automatic blood analyzer.
In the radon group, the numbers of CD3 ( = -0.140, > .05) and CD4CD8 ( = -0.964, > .05) T cells were higher, as compared with the controls, but this difference was not significant. In addition, the number of CD4CD8 ( = -2.141, < .05) T cells was significantly lower in the radon group. Furthermore, the average ratios of CD4/CD8 ( = -2.201, < .05) and TCR/CD3 ( = 2.047, < .05) cells were significantly higher in the radon group than in the controls. Compared with the control group, the LYMPH# ( = -0.485, > .05) and LYMPH% (Z = -0.835, > .05) showed no significant change.
Radon-rich hot springs could alter the proportions of lymphocyte subsets and possibly affect immunologic functions.
通过检测温泉附近居民外周血淋巴细胞亚群来分析免疫功能的变化。
随机选取两组；将61名温泉附近居民归入氡气组，51名生活方式和习惯相似但未接触温泉的居民归入对照组。在FACS Aria流式细胞仪上评估两组淋巴细胞亚群（CD3、CD4CD8、CD4CD8、CD4/CD8和TCR/CD3）的百分比。用自动血液分析仪测量淋巴细胞绝对值（LYMPH#）和淋巴细胞百分比（LYMPH%）。
与对照组相比，氡气组CD3（=-0.140，>.05）和CD4CD8（=-0.964，>.05）T细胞数量较高，但差异不显著。此外，氡气组CD4CD8（=-2.141，<.05）T细胞数量显著较低。此外，氡气组CD4/CD8（=-2.201，<.05）和TCR/CD3（=2.047，<.05）细胞的平均比率显著高于对照组。与对照组相比，LYMPH#（=-0.485，>.05）和LYMPH%（Z=-0.835，>.05）无显著变化。
富含氡气的温泉可能会改变淋巴细胞亚群比例，并可能影响免疫功能。
