Institut Pasteur, INRA, Unité Biologie et Pathogénicité Fongiques, Paris, France.
Université Paris Diderot, Sorbonne Paris Cité, Paris, France.
Antimicrob Agents Chemother. 2019 Apr 25;63(5). doi: 10.1128/AAC.01979-18. Print 2019 May.
is known for its ability to form biofilms, which are communities of microorganisms embedded in an extracellular matrix developing on different surfaces. Biofilms are highly tolerant to antifungal therapy. This phenomenon has been partially explained by the appearance of so-called persister cells, phenotypic variants of wild-type cells, capable of surviving very high concentrations of antimicrobial agents. Persister cells in were found exceptionally in biofilms, while none were detected in planktonic cultures of this fungus. Yet, this topic remains controversial, as others could not observe persister cells in biofilms formed by the SC5314 laboratory strain. Due to ambiguous data in the literature, this work aimed to reevaluate the presence of persister cells in biofilms. We demonstrated that the isolation of "persister cells" as described previously was likely to be the result of the survival of biofilm cells that were not reached by the antifungal. We tested biofilms of SC5314 and its derivatives, as well as 95 clinical isolates, using an improved protocol, demonstrating that persister cells are not a characteristic trait of biofilms. Although some clinical isolates are able to yield survivors upon the antifungal treatment of biofilms, this phenomenon is rather stochastic and inconsistent.
它以形成生物膜的能力而闻名，生物膜是微生物群落嵌入在不同表面上的细胞外基质中发展而成的。生物膜对抗真菌治疗具有高度耐受性。这种现象部分可以通过所谓的持久细胞的出现来解释，持久细胞是野生型细胞的表型变体，能够在非常高浓度的抗菌剂中存活。在 生物膜中发现了异常的持久细胞，而在该真菌的浮游培养物中则没有检测到。然而，这个话题仍然存在争议，因为其他人在由 SC5314 实验室菌株形成的生物膜中无法观察到持久细胞。由于文献中的数据不明确，这项工作旨在重新评估 生物膜中是否存在持久细胞。我们证明，如前所述分离“持久细胞”可能是由于未被抗真菌药物触及的生物膜细胞的存活所致。我们使用改进的方案测试了 SC5314 及其衍生物以及 95 个临床分离株的生物膜，证明持久细胞不是 生物膜的特征特征。尽管一些临床分离株在抗真菌治疗生物膜时能够产生存活者，但这种现象是随机且不一致的。
