Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD, 20705, USA.
Institute of Biological Chemistry, Washington State University, Pullman, WA, 99164, USA.
Proteomics. 2019 Apr;19(7):e1800379. doi: 10.1002/pmic.201800379.
To understand the effect of fatty acid desaturase gene (GmFAD3) silencing on perturbation of fatty acid (FA) metabolic pathways, the changes are compared in protein profiling in control and low linolenic acid transgenic soybeans using tandem mass tag based mass spectrometry. Protein profiling of the transgenic line unveiled changes in several key enzymes of FA metabolism. This includes enzymes of lower abundance; fabH, fabF, and thioestrase associated with FA initiation, elongation, and desaturation processes and LOX1_5, ACOX, ACAA1, MFP2 associated with β-oxidation of α-linolenic acids pathways. In addition, the GmFAD3 silencing results in a significant reduction in one of the major allergens, Gly m 4 (C6T3L5). These results are important for exploring how plants adjust in their biological processes when certain changes are induced in the genetic makeup. A complete understanding of these processes will aid researchers to alter genes for developing value-added soybeans.
为了了解脂肪酸去饱和酶基因（GmFAD3）沉默对脂肪酸（FA）代谢途径扰动的影响，我们使用基于串联质量标签的质谱比较了对照和低亚麻酸转基因大豆中蛋白质谱的变化。转基因株系的蛋白质谱揭示了 FA 代谢的几个关键酶的变化。这包括丰度较低的酶；fabH、fabF 和硫酯酶与 FA 的起始、延伸和去饱和过程有关，而 LOX1_5、ACOX、ACAA1 和 MFP2 与 α-亚麻酸途径的β-氧化有关。此外，GmFAD3 的沉默导致一种主要过敏原 Gly m 4（C6T3L5）的显著减少。这些结果对于探索当遗传组成发生某些变化时，植物如何在其生物过程中进行调整是很重要的。对这些过程的全面了解将有助于研究人员改变基因，以开发高附加值的大豆。
