Institute of Microbiology and Biotechnology, Technical University of Moldova, 2028 Chisinau, Moldova.
Mar Drugs. 2024 May 1;22(5):208. doi: 10.3390/md22050208.
The purpose of this study was to examine the influence of 10 and 20 nm nanoparticles (AgNPs) on the growth and biochemical composition of microalga CNMN-AR-02 in two media which differ by the total amount of mineral salts (MM1 with 33.02 g/L and MM2 with 21.65 g/L). Spectrophotometric methods were used to estimate the amount of biomass and its biochemical composition. This study provides evidence of both stimulatory and inhibitory effects of AgNPs on different parameters depending on the concentration, size, and composition of the nutrient medium. In relation to the mineral medium, AgNPs exhibited various effects on the content of proteins (an increase up to 20.5% in MM2 and a decrease up to 36.8% in MM1), carbohydrates (a decrease up to 35.8% in MM1 and 39.6% in MM2), phycobiliproteins (an increase up to 15.7% in MM2 and 56.8% in MM1), lipids (an increase up to 197% in MM1 and no changes found in MM2), antioxidant activity (a decrease in both media). The composition of the cultivation medium has been revealed as one of the factors influencing the involvement of nanoparticles in the biosynthetic activity of microalgae.
本研究旨在研究 10nm 和 20nm 纳米颗粒（AgNPs）对两种不同总盐量（MM1 为 33.02g/L，MM2 为 21.65g/L）培养基中微藻 CNMN-AR-02 的生长和生化成分的影响。采用分光光度法估算生物量及其生化成分。本研究证明了 AgNPs 对不同参数的刺激和抑制作用取决于浓度、大小和营养培养基的组成。就矿物培养基而言，AgNPs 对蛋白质含量（在 MM2 中增加高达 20.5%，在 MM1 中减少高达 36.8%）、碳水化合物（在 MM1 中减少高达 35.8%，在 MM2 中减少高达 39.6%）、藻胆蛋白（在 MM2 中增加高达 15.7%，在 MM1 中增加高达 56.8%）、脂质（在 MM1 中增加高达 197%，在 MM2 中没有变化）、抗氧化活性（在两种培养基中均降低）有不同的影响。培养介质的组成被揭示为影响纳米颗粒参与微藻生物合成活性的因素之一。
