Rus Ciucă D, Soriţău Olga, Suşman S, Pop V I, Mihu Carmen Mihaela
Department of Histology, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania.
Rom J Morphol Embryol. 2011;52(3):803-8.
The aim of the study was the isolation and characterization of mesenchymal stem cells from the placental chorion from a genotypical and phenotypical point of view. The placentas included in the study were derived from term pregnancies with a normal evolution. Along with the placentas, umbilical cord blood, maternal and newborn peripheral blood samples were taken. The isolation and culture of chorionic and, incidentally, trophoblastic cells was followed by the determination of markers of the former cells. They expressed proteins and genes characteristic of stem cells. Immunofluorescence and evaluation of gene expression evidenced the pluripotential properties of these cells and also their higher position on the differentiation pathway. HLA expression provides information that might help explain the immunological mechanisms of tolerance between the maternal organism and fetal structures.
本研究的目的是从基因型和表型的角度分离和鉴定胎盘绒毛膜间充质干细胞。纳入研究的胎盘来自足月妊娠且孕期进展正常。除胎盘外，还采集了脐带血、母体和新生儿外周血样本。在分离和培养绒毛膜细胞以及偶然的滋养层细胞后，对前者细胞的标志物进行了测定。它们表达干细胞特有的蛋白质和基因。免疫荧光和基因表达评估证明了这些细胞的多能特性以及它们在分化途径上的更高位置。HLA表达提供的信息可能有助于解释母体机体与胎儿结构之间耐受的免疫机制。
