Pham Maria H, Haugen Håvard J, Reseland Janne E
Department of Biomaterials, Institute of Clinical Dentistry, University of Oslo, 0317 Oslo, Norway.
Materials (Basel). 2020 Feb 3;13(3):684. doi: 10.3390/ma13030684.
Immediately after dental implant insertion, blood will be in direct contact and interact with the implant surface and activates inflammatory responses and complement cascades within seconds. The aim of the present study was to determine the ability of fluoride-modified titanium surfaces to activate complement cascades using the human buffy coat as model. The buffy coats were exposed to hydrofluoric acid-modified surfaces for a short time and its responses were compared to controls. Identification and quantification of complement cascade biomarkers were conducted using ELISA kits and multianalyte profiling using Luminex. A lower level of C3 at 30 min and increased levels of C4, MIP-4, CRP, and pigment epithelium-derived factor at 360 min were found on modified surfaces as compared to controls. We found no significant differences in the levels of C3a, C5a, C Factor H, α2M, ApoA1, ApoC3, ApoE, Prealbumin, α1AT, and SAP in modified surfaces in the buffy coats. We conclude that titanium surfaces treated with hydrofluoric acid modify the levels of specific biomarkers related to the complement cascade and angiogenesis and, thus, tissue growth, remodeling and repair, as this may play a role in the enhanced clinical performance of fluoride-modified Ti dental implants.
牙种植体植入后，血液会立即与种植体表面直接接触并相互作用，在数秒内激活炎症反应和补体级联反应。本研究的目的是以人血沉棕黄层为模型，确定氟改性钛表面激活补体级联反应的能力。将血沉棕黄层短时间暴露于氢氟酸改性表面，并将其反应与对照组进行比较。使用酶联免疫吸附测定试剂盒对补体级联生物标志物进行鉴定和定量，并使用Luminex进行多分析物分析。与对照组相比，改性表面在30分钟时C3水平较低，在360分钟时C4、MIP-4、CRP和色素上皮衍生因子水平升高。我们发现血沉棕黄层中改性表面的C3a、C5a、C因子H、α2M、载脂蛋白A1、载脂蛋白C3、载脂蛋白E、前白蛋白、α1抗胰蛋白酶和血清淀粉样蛋白P水平没有显著差异。我们得出结论，氢氟酸处理的钛表面会改变与补体级联反应和血管生成相关的特定生物标志物的水平，从而影响组织生长、重塑和修复，因为这可能在氟改性钛牙种植体的临床性能增强中发挥作用。
