Shinada M, Azuma M, Kawai H, Sazaki K, Yoshida I, Yoshida T, Suzutani T, Sakuma T
Proc Soc Exp Biol Med. 1986 Feb;181(2):205-10. doi: 10.3181/00379727-181-42241.
The effects of glycyrrhizin, a component of licorice (Glycyrrhiza glabra) roots, on the production of interferon-gamma in human peripheral lymphocyte-macrophage cultures by concanavalin A (Con A) was examined. Interferon-gamma production in normal lymphocyte-macrophage cultures treated with 10 to 100 micrograms/ml of glycyrrhizin at 37 degrees C for 12 hr or longer, and then treated with 10 micrograms/ml of Con A, was enhanced four to eight times compared to control cell cultures. Lymphocyte-macrophage cultures treated with 10 to 100 micrograms/ml of glycyrrhizin alone did not produce interferon. No significant difference in the adsorption of [3H]Con A to glycyrrhizin-treated and control lymphocyte-macrophage cultures was found, but RNA and protein synthesis of the treated lymphocytes was increased compared to control cells; DNA synthesis, however, was reduced. Collaboration between enriched T-lymphocytes and macrophages, both treated with glycyrrhizin, was needed for the enhancement of interferon-gamma production. A smaller increase in interferon production was also observed in the glycyrrhizin-treated peripheral lymphocyte-macrophage cultures derived from an asymptomatic carrier of hepatitis B virus, in response to Con A and surface antigen of hepatitis B virus.
研究了甘草（Glycyrrhiza glabra）根中的成分甘草甜素对伴刀豆球蛋白A（Con A）刺激人外周淋巴细胞 - 巨噬细胞培养物中γ干扰素产生的影响。在37℃下用10至100微克/毫升甘草甜素处理正常淋巴细胞 - 巨噬细胞培养物12小时或更长时间，然后用10微克/毫升Con A处理，与对照细胞培养物相比，γ干扰素的产生增强了4至8倍。单独用10至100微克/毫升甘草甜素处理的淋巴细胞 - 巨噬细胞培养物不产生干扰素。未发现[3H]Con A与甘草甜素处理的和对照淋巴细胞 - 巨噬细胞培养物的吸附有显著差异，但与对照细胞相比，处理后淋巴细胞的RNA和蛋白质合成增加；然而，DNA合成减少。为增强γ干扰素的产生，需要富集的T淋巴细胞和巨噬细胞都用甘草甜素处理并进行协作。在来自无症状乙肝病毒携带者的经甘草甜素处理的外周淋巴细胞 - 巨噬细胞培养物中，对Con A和乙肝病毒表面抗原的反应中，也观察到干扰素产生的较小增加。
