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内体分选转运复合体（ESCRT）复合物的组分相互作用对于[具体物种]中内吞作用依赖的生长、繁殖、脱氧雪腐镰刀菌烯醇（DON）产生及完全毒力至关重要。

Component Interaction of ESCRT Complexes Is Essential for Endocytosis-Dependent Growth, Reproduction, DON Production and Full Virulence in .

作者信息

Xie Qiurong, Chen Ahai, Zhang Yunzhi, Yuan Mingyue, Xie Wei, Zhang Chengkang, Zheng Wenhui, Wang Zonghua, Li Guangpu, Zhou Jie

机构信息

Key Laboratory of Biopesticide and Chemical Biology of Education Ministry, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, China.

Fujian University Key Laboratory for Plant-Microbe Interaction, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.

出版信息

Front Microbiol. 2019 Feb 12;10:180. doi: 10.3389/fmicb.2019.00180. eCollection 2019.

DOI:10.3389/fmicb.2019.00180
PMID:30809208
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6379464/
Abstract

Multivesicular bodies (MVBs) are critical intermediates in the trafficking of ubiquitinated endocytosed surface proteins to the lysosome/vacuole for destruction. Recognizing and packaging ubiquitin modified cargoes to the MVB pathway require ESCRT (Endosomal sorting complexes required for transport) machinery, which consists of four core subcomplexes, ESCRT-0, ESCRT-I, ESCRT-II, and ESCRT-III. is an important plant pathogen that causes head blight of major cereal crops. Our previous results showed that ESCRT-0 is essential for fungal development and pathogenicity in . We then, in this study, systemically studied the protein-protein interactions within ESCRT-I, -II or -III complex, as well as between ESCRT-0 and ESCRT-I, ESCRT-I and ESCRT-II, and ESCRT-II and ESCRT-III complexes and found that loss of any ESCRT component resulted in abnormal function in endocytosis. In addition, ESCRT deletion mutants displayed severe defects in growth, deoxynivalenol (DON) production, virulence, sexual, and asexual reproduction. Importantly genetic complementation with corresponding ESCRT genes fully rescued all these defective phenotypes, indicating the essential role of ESCRT machinery in fungal development and plant infection in . Taken together, the protein-protein interactome and biological functions of the ESCRT machinery is first profoundly characterized in , providing a foundation for further exploration of ESCRT machinery in filamentous fungi.

摘要

多囊泡体（MVBs）是泛素化的内吞表面蛋白转运至溶酶体/液泡进行降解过程中的关键中间体。识别泛素修饰的货物并将其包装到MVB途径需要ESCRT（转运所需的内体分选复合体）机制，该机制由四个核心亚复合体ESCRT-0、ESCRT-I、ESCRT-II和ESCRT-III组成。 是一种重要的植物病原体，可导致主要谷类作物的赤霉病。我们之前的结果表明，ESCRT-0对于 中的真菌发育和致病性至关重要。然后，在本研究中，我们系统地研究了ESCRT-I、-II或-III复合体内以及ESCRT-0与ESCRT-I、ESCRT-I与ESCRT-II以及ESCRT-II与ESCRT-III复合体之间的蛋白质-蛋白质相互作用，发现任何ESCRT组分的缺失都会导致内吞作用功能异常。此外，ESCRT缺失突变体在生长、脱氧雪腐镰刀菌烯醇（DON）产生、毒力、有性和无性繁殖方面表现出严重缺陷。重要的是，用相应的ESCRT基因进行遗传互补完全挽救了所有这些缺陷表型，表明ESCRT机制在 中的真菌发育和植物感染中起着至关重要的作用。综上所述，首次在 中深入表征了ESCRT机制的蛋白质-蛋白质相互作用组和生物学功能，为进一步探索丝状真菌中的ESCRT机制奠定了基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c873/6379464/a622b510046f/fmicb-10-00180-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c873/6379464/29317f1aa973/fmicb-10-00180-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c873/6379464/171ac1eea50c/fmicb-10-00180-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c873/6379464/32dfaadeaf4d/fmicb-10-00180-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c873/6379464/68e90cd2fa89/fmicb-10-00180-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c873/6379464/239193e672c5/fmicb-10-00180-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c873/6379464/f04de5cae76a/fmicb-10-00180-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c873/6379464/a622b510046f/fmicb-10-00180-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c873/6379464/29317f1aa973/fmicb-10-00180-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c873/6379464/171ac1eea50c/fmicb-10-00180-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c873/6379464/32dfaadeaf4d/fmicb-10-00180-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c873/6379464/68e90cd2fa89/fmicb-10-00180-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c873/6379464/239193e672c5/fmicb-10-00180-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c873/6379464/f04de5cae76a/fmicb-10-00180-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c873/6379464/a622b510046f/fmicb-10-00180-g0007.jpg

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