Tvarijonaviciute A, Barić-Rafaj R, Horvatic A, Muñoz-Prieto A, Guillemin N, Lamy E, Tumpa A, Ceron J J, Martinez-Subiela S, Mrljak V
Interdisciplinary Laboratory of Clinical Pathology, Interlab-UMU, Campus of Excellence Mare Nostrum, University of Murcia, Murcia, Spain.
Department of Chemistry and Biochemistry, Faculty of Veterinary Medicine, University of Zagreb, 10000 Zagreb, Croatia.
Vet J. 2019 Feb;244:51-59. doi: 10.1016/j.tvjl.2018.12.006. Epub 2018 Dec 13.
The main objective of this study was to identify analytes that could change and that could help to clarify the metabolic and physiopathological changes related to canine obesity-related metabolic dysfunction (ORMD). For this, serum from 35 overweight/obese dogs, with and without ORMD, was submitted to a comprehensive panel of biochemistry analysis, a gel-free tandem mass tag isobaric label-based proteomic analysis, and, finally, selected proteins were used as a starting point for creating a protein interaction network. Dogs with ORMD showed significantly higher serum concentrations of alanine aminotransferase (ALT), alkaline phosphatase (ALP), Ca, total proteins, albumin, total cholesterol, triglycerides, glucose, and butyrylcholinesterase (BChE) activity in comparison with dogs without ORMD. Proteomic analysis revealed that 23 proteins related to lipid metabolism, the complement factor system, cellular adhesion and functionality, inflammation, and coagulation were altered in dogs with ORMD. Finally, the obtained protein interaction network highlighted that the central term of this network was the negative regulation of the immune response. These data suggest that canine ORMD is associated with changes in analytes that reflect altered lipid metabolism, and liver and immune function impairment and suggests the potential for a prothrombotic state and lung function alterations.
本研究的主要目的是识别可能发生变化的分析物，这些分析物有助于阐明与犬类肥胖相关代谢功能障碍（ORMD）相关的代谢和生理病理变化。为此，从35只超重/肥胖犬（有或无ORMD）采集血清，进行全面的生物化学分析、基于无凝胶串联质量标签等压标记的蛋白质组学分析，最后，将选定的蛋白质作为构建蛋白质相互作用网络的起点。与无ORMD的犬相比，患有ORMD的犬血清中丙氨酸氨基转移酶（ALT）、碱性磷酸酶（ALP）、钙、总蛋白、白蛋白、总胆固醇、甘油三酯、葡萄糖和丁酰胆碱酯酶（BChE）活性显著更高。蛋白质组学分析显示，与脂质代谢、补体因子系统、细胞黏附和功能、炎症及凝血相关的23种蛋白质在患有ORMD的犬中发生了改变。最后，所获得的蛋白质相互作用网络突出显示，该网络的核心术语是免疫反应的负调控。这些数据表明，犬类ORMD与反映脂质代谢改变、肝脏和免疫功能受损的分析物变化相关，并提示存在血栓形成倾向和肺功能改变的可能性。
