Xiong Wenxiang, Liang Yaru, Li Xue, Liu Guosong, Wang Zhao
MOE Key Laboratory of Protein Sciences, School of Pharmaceutical Sciences, Tsinghua University.
Center for Drug Evaluation, National Medical Products Administration.
Biol Pharm Bull. 2019;42(3):357-364. doi: 10.1248/bpb.b18-00406.
Magnesium (Mg) is an endogenous cation that is involved in many essential biological reactions. Abnormal Mg metabolisms in the body affect important physiological and pathological processes. However, most endogenous Mg markers fail to represent body Mg status; they are disadvantageous in terms of representational capacity, applied range, operational convenience, etc. In this article, we evaluated some of the most popular Mg marker candidates. A logical model of the blood Mg compartments was established, which consisted of unstable Mg pools, representative Mg pools, and conserved Mg pools. These pools were based on the metabolic efficiency of Mg in an acute Mg intake test. The results of this study showed that only the erythrocyte intracellular ionized Mg (RBC [Mg]), a representative Mg pool, could effectively represent abnormal body Mg metabolisms in various conditions, including dietary Mg adjustments, aging and metabolic syndrome. These results suggest that RBC [Mg] might be a widely applicable marker of body Mg levels. On unified technology platform and evaluation system, this research compared the representative capacities of RBC [Mg], plasma Mg concentration (plasma [Mg]), erythrocyte intracellular total Mg (RBC [Mg]) and Mg retention in rats and mice under various Mg-metabolism-related physiological and pathological conditions. Our technique for the direct quantitative analysis of RBC [Mg] may prove valuable for basic physiological research, dietary Mg regulation, as well as clinical monitoring/intervention of Mg-metabolism-related pathology.
镁（Mg）是一种内源性阳离子，参与许多重要的生物反应。体内镁代谢异常会影响重要的生理和病理过程。然而，大多数内源性镁标志物无法反映身体的镁状态；它们在代表性、应用范围、操作便利性等方面存在劣势。在本文中，我们评估了一些最常用的镁标志物候选物。建立了一个血液镁区室的逻辑模型，该模型由不稳定镁池、代表性镁池和保守镁池组成。这些池是基于急性镁摄入试验中镁的代谢效率划分的。本研究结果表明，只有红细胞内游离镁（RBC [Mg]），即一个代表性镁池，能够在包括饮食镁调整、衰老和代谢综合征等各种情况下有效反映身体异常的镁代谢。这些结果表明，RBC [Mg]可能是一种广泛适用的身体镁水平标志物。在统一的技术平台和评估系统上，本研究比较了RBC [Mg]、血浆镁浓度（plasma [Mg]）、红细胞内总镁（RBC [Mg]）以及在各种与镁代谢相关的生理和病理条件下大鼠和小鼠体内镁潴留的代表性能力。我们直接定量分析RBC [Mg]的技术可能对基础生理学研究、饮食镁调节以及镁代谢相关病理学的临床监测/干预具有重要价值。
