Reis Rengin, Sipahi Hande, Zeybekoğlu Gülşah, Çelik Nur, Kırmızıbekmez Hasan, Kaklıkkaya Neşe, Aydın Ahmet
Department of Toxicology, Yeditepe University, Istanbul, Turkey.
Department of Toxicology, Karadeniz Technical University, Trabzon, Turkey.
Euroasian J Hepatogastroenterol. 2018 Jul-Dec;8(2):126-132. doi: 10.5005/jp-journals-10018-1278. Epub 2019 Feb 1.
The fruits of L. is widely consumed as food, and olive pits are utilized in folk medicine to relieve gastric disturbances. In the present study, the possible anti-inflammatory, analgesic and antioxidant activities of aqueous extracts of black (BP) and green olive (GP) pit prepared at gastric fed state pH were evaluated Moreover, the bioactive compound, hydroxytyrosol (HT), was isolated from the extracts for the first time. According to results, GP extract (62.5 to 1000 μg/mL) showed significant anti-inflammatory activity in a dose-dependent manner and HT displayed significant nitrite inhibition at 100 μM with slight analgesic activity. Extracts and HT showed a significant antioxidant activity according to Total Antioxidant Capacity (TOAC), cupric ion reducing antioxidant capacity (CUPRAC), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays. As a conclusion, a proper formulation containing HT might be a potential remedy to relieve gastric disturbances and olive pits, can be utilized as a valuable industrial tool for the low-cost production of HT. Reis R, Sipahi H, Zeybekoglu G, Celik N, Kirmizibekmez H, Kaklikkaya N, Aydin A. Hydroxytyrosol: The Factor Responsible for Bioactivity of Traditionally used Olive Pits. Euroasian J Hepatogastroenterol, 2018;8(2):126-132.
油橄榄的果实被广泛用作食物，橄榄核在民间医学中被用于缓解胃部不适。在本研究中，评估了在胃内喂食状态pH下制备的黑橄榄（BP）和绿橄榄（GP）核水提取物可能的抗炎、镇痛和抗氧化活性。此外，首次从提取物中分离出生物活性化合物羟基酪醇（HT）。根据结果，GP提取物（62.5至1000μg/mL）呈剂量依赖性地显示出显著的抗炎活性，HT在100μM时显示出显著的亚硝酸盐抑制作用，并具有轻微的镇痛活性。根据总抗氧化能力（TOAC）、铜离子还原抗氧化能力（CUPRAC）和2,2-二苯基-1-苦基肼（DPPH）自由基清除试验，提取物和HT显示出显著的抗氧化活性。总之，含有HT的合适制剂可能是缓解胃部不适的潜在药物，橄榄核可作为低成本生产HT的有价值工业原料。雷斯R、锡帕希H、泽伊贝科格鲁G、塞利克N、基尔米齐贝克梅兹H、卡克利卡亚N、艾登A。羟基酪醇：传统使用的橄榄核生物活性的 responsible因素。《欧亚肝脏胃肠病学杂志》，2018年；8（2）：126 - 132。 （注：最后一句中“responsible”前似乎少了个词，翻译时保留了原文的不完整性）
