Department of Pharmacology, Faculty of Pharmacy, University of Health Sciences, 34668 Istanbul, Turkey.
Department of Pharmacognosy, School of Pharmacy, Istanbul Medipol University, 34810 Istanbul, Turkey.
Molecules. 2020 Jun 10;25(11):2695. doi: 10.3390/molecules25112695.
The air-dried aerial parts of (Sims) Lag. Ex Benth. was extracted by methanol and fractionated by -hexane, dichloromethane, and ethyl acetate, respectively. The wound healing properties of extract gel was evaluated using the in vivo excisional wound model using Balb-c mice. Initially, the methanol extract showed LOX inhibitory activity at IC = 23.2 µg/mL, whereas the DPPH assay showed IC = 0.89 mg/mL, and the ABTS assay showed IC = 0.99 mg/mL, respectively. In addition, a remarkable anti-inflammatory activity was observed in the cell culture assay. Thereafter, activity-guided fractionation was performed by LOX enzyme inhibition assays, and the structures of the two most active fractions were revealed by both GC-FID and GC/MS analyses, simultaneously. Phytol and 1-heptadecanoic acid were characterized as the active constituents. Moreover, the extract gel formulation was applied for in vivo tests, where the new gel formulation supported the in vitro anti-inflammatory activity findings. As a conclusion, this experimental results support the wound healing evidence based on the ethnobotanical application of species with further potential.
（Sims）Lag. Ex Benth. 的风干地上部分用甲醇提取，分别用正己烷、二氯甲烷和乙酸乙酯进行萃取和分级。使用 Balb-c 小鼠的体内切口模型评估 提取物凝胶的伤口愈合特性。最初，甲醇提取物在 IC = 23.2 µg/mL 时表现出 LOX 抑制活性，而 DPPH 测定法显示 IC = 0.89 mg/mL，ABTS 测定法显示 IC = 0.99 mg/mL。此外，在细胞培养测定中观察到显著的抗炎活性。此后，通过 LOX 酶抑制测定进行活性导向的分级，并用 GC-FID 和 GC/MS 分析同时揭示了两个最活跃的馏分的结构。植醇和 1-十七烷酸被鉴定为活性成分。此外，提取物凝胶制剂用于体内测试，新的凝胶制剂支持体外抗炎活性的发现。总之，这些实验结果支持基于该物种的基于民族植物学的伤口愈合证据，具有进一步的潜力。
