Department of Plant Science, Bharathidasan University, Tiruchirappalli, Tamil Nadu 24, India.
Department of Plant Science, Bharathidasan University, Tiruchirappalli, Tamil Nadu 24, India.
Carbohydr Polym. 2016 Oct 20;151:321-325. doi: 10.1016/j.carbpol.2016.05.033. Epub 2016 May 13.
The aim of the present study was to prepare Chitosan nanoparticles through biological method with high antifungal activities. Chitosan nanoparticles were prepared by the addition of anionic proteins isolated from Penicillium oxalicum culture to chitosan solutions. The formation of chitosan nanoparticles was preliminary confirmed by UV-vis spectrophotometric analysis. The physico-chemical properties of the chitosan nanoparticles were determined by size and zeta potential analysis, FTIR analysis, HRTEM and XRD pattern. The chitosan nanoparticles were evaluated for its potential to inhibit the growth of phytopathogens viz., Pyricularia grisea, Alternaria solani, Fusarium oxysporum. It is evident from our results that chitosan nanoparticles inhibit the growth of phytopathogens tested. Chitosan nanoparticle treated chickpea seeds showed positive morphological effects such as enhanced germination%, seed vigor index and vegetative biomass of seedlings. All these results indicate that chitosan nanoparticle can be used further under field condition to protect various crops from the devastating fungal pathogens as well as growth promoters.
本研究旨在通过生物方法制备具有高抗真菌活性的壳聚糖纳米粒子。通过向壳聚糖溶液中添加从草酸青霉培养物中分离出的阴离子蛋白来制备壳聚糖纳米粒子。通过紫外可见分光光度分析初步证实了壳聚糖纳米粒子的形成。通过粒径和zeta 电位分析、FTIR 分析、HRTEM 和 XRD 图谱来确定壳聚糖纳米粒子的物理化学性质。评估壳聚糖纳米粒子抑制植物病原菌（稻瘟病菌、茄丝核菌、尖孢镰刀菌）生长的潜力。从我们的结果可以明显看出，壳聚糖纳米粒子抑制了所测试的植物病原菌的生长。用壳聚糖纳米粒子处理的鹰嘴豆种子表现出积极的形态效应，如提高发芽率%、种子活力指数和幼苗的营养生物量。所有这些结果表明，壳聚糖纳米粒子可以在田间条件下进一步用于保护各种作物免受破坏性真菌病原体以及生长促进剂的侵害。
