College of Food Science & Technology, Shanghai Ocean University, No. 999 Huchenghuan Road, Shanghai 201306, China.
ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, VIC 3052, Australia.
J Biosci Bioeng. 2019 Aug;128(2):234-240. doi: 10.1016/j.jbiosc.2019.02.004. Epub 2019 Mar 2.
Collagen plays a pivotal role in human physiological functions and extracted collagen has multiple potential applications. Tilapia skin can be applied to extract collagen for maximizing the profit of tilapia processing. Electrospinning/electrospraying is novel micro- and nano-techniques to fabricate microfibers and microspheres in a simple and easy way. In this work, we extract collagens from tilapia skin by three types of extraction methods: acetic acid method, hot water method, and sodium hydroxide method. Then, these extracted collagens are characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Fourier transform infrared spectrometry. These extracted collagens have different molecular weights and different protein secondary structures. Finally, these extracted collagens are applied to fabricate electrosprayed microspheres, electrospun microfibers, and mixed microspheres/microfibers with multiple potential applications by adjusting the collagen concentrations. Higher polymer molecular weight only needs lower concentration to produce microfibers. The microfiber diameter increases with the increase of collagen concentration. This work proves that extraction methods have obvious effect on the preparation of electrospun/electrosprayed microstructures of tilapia skin collagen and provide a way to maximize resource utilization of tilapia processing waste.
胶原蛋白在人类生理功能中起着关键作用，提取的胶原蛋白具有多种潜在应用。罗非鱼皮可用于提取胶原蛋白，以最大限度地提高罗非鱼加工的利润。静电纺丝/静电喷雾是一种新颖的微纳技术，可简单、轻松地制造微纤维和微球。在这项工作中，我们通过三种提取方法：乙酸法、热水法和氢氧化钠法，从罗非鱼皮中提取胶原蛋白。然后，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和傅里叶变换红外光谱对提取的胶原蛋白进行表征。这些提取的胶原蛋白具有不同的分子量和不同的蛋白质二级结构。最后，通过调整胶原蛋白浓度，将这些提取的胶原蛋白应用于制备电喷雾微球、静电纺丝微纤维以及具有多种潜在应用的混合微球/微纤维。较高的聚合物分子量只需要较低的浓度即可产生微纤维。微纤维的直径随胶原蛋白浓度的增加而增加。这项工作证明了提取方法对罗非鱼皮胶原蛋白静电纺丝/静电喷雾微结构制备有明显影响，并为最大限度地利用罗非鱼加工废物提供了一种方法。
