Al-Habib Mey, Huang George T-J
Faculty of Dentistry, Department of Endodontics, King Abdulaziz University, Jeddah, Saudi Arabia.
Department of Bioscience Research, University of Tennessee Health Science Center, College of Dentistry, Memphis, TN, USA.
Methods Mol Biol. 2019;1922:59-76. doi: 10.1007/978-1-4939-9012-2_7.
Dental stem cells (DSCs) have been shown to possess great potential for multiple biomedical applications, especially for dental tissue regeneration. They are a special type of subpopulation of mesenchymal stem/stromal cells (MSCs) and present subtle differences from other types of MSCs. Therefore, it requires a specialized expertise to isolate, culture, and characterize these cells in vitro and in vivo. The purpose of this chapter is to share our experience in studying these cells. We will describe in detail laboratory protocols outlining how the cells are isolated, cultured, expanded, and characterized using various in vitro cellular and biochemical analyses, as well as an in vivo study model using immunocompromised mice to observe tissue regeneration after transplantation of these DSCs.
牙干细胞（DSCs）已被证明在多种生物医学应用中具有巨大潜力，尤其是在牙组织再生方面。它们是间充质干/基质细胞（MSCs）的一种特殊亚群，与其他类型的MSCs存在细微差异。因此，在体外和体内分离、培养和鉴定这些细胞需要专业知识。本章的目的是分享我们研究这些细胞的经验。我们将详细描述实验室方案，概述如何使用各种体外细胞和生化分析方法分离、培养、扩增和鉴定这些细胞，以及使用免疫缺陷小鼠的体内研究模型来观察这些DSCs移植后的组织再生情况。
