National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
Agronomy, Horticulture and Plant Sciences Dept., South Dakota State University, Brookings, South Dakota, United States of America.
PLoS One. 2019 Mar 6;14(3):e0212671. doi: 10.1371/journal.pone.0212671. eCollection 2019.
In this study, high-throughput sequencing (RNA-Seq) was utilized to evaluate differential expression of transcripts and their related genes involved in response to terminal drought in root tissues of bread wheat landrace (L-82) and drought-sensitive genotype (Marvdasht). Subsets of 460 differentially expressed genes (DEGs) in drought-tolerant genotype and 236 in drought-sensitive genotype were distinguished and functionally annotated with 105 gene ontology (GO) terms and 77 metabolic pathways. Transcriptome profiling of drought-resistant genotype "L-82" showed up-regulation of genes mostly involved in Oxidation-reduction process, secondary metabolite biosynthesis, abiotic stress response, transferase activity and heat shock proteins. On the other hand, down-regulated genes mostly involved in signaling, oxidation-reduction process, secondary metabolite biosynthesis, auxin-responsive protein and lipid metabolism. We hypothesized that the drought tolerance in "L-82" was a result of avoidance strategies. Up-regulation of genes related to the deeper root system and adequate hydraulic characteristics to allow water uptake under water scarcity confirms our hypothesis. The transcriptomic sequences generated in this study provide information about mechanisms of acclimation to drought in the selected bread wheat landrace, "L-82", and will help us to unravel the mechanisms underlying the ability of crops to reproduce and keep its productivity even under drought stress.
在这项研究中，我们利用高通量测序（RNA-Seq）来评估转录本的差异表达及其相关基因，这些基因涉及到面包小麦地方品种（L-82）和干旱敏感基因型（Marvdasht）根系组织对终末干旱的反应。在耐旱基因型中区分出了 460 个差异表达基因（DEGs）亚组，在干旱敏感基因型中区分出了 236 个，这些基因都具有 105 个基因本体论（GO）术语和 77 个代谢途径的功能注释。耐旱基因型“L-82”的转录组分析表明，参与氧化还原过程、次生代谢物生物合成、非生物胁迫反应、转移酶活性和热休克蛋白的基因上调。另一方面，下调的基因主要涉及信号转导、氧化还原过程、次生代谢物生物合成、生长素响应蛋白和脂质代谢。我们假设“L-82”的耐旱性是一种避免策略的结果。与更深的根系系统和充足的水力特性相关的基因的上调，使植物在缺水时能够吸收水分，这证实了我们的假设。本研究中生成的转录组序列提供了关于所选面包小麦地方品种“L-82”适应干旱的机制信息，这将帮助我们揭示作物在干旱胁迫下繁殖和保持生产力的能力的机制。
