Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA.
Program in Immunology, Sackler School of Biomedical Sciences, Tufts University School of Medicine, Boston, MA.
Microbiol Spectr. 2019 Mar;7(2). doi: 10.1128/microbiolspec.BAI-0013-2019.
Over the past few decades, cell culture systems have greatly expanded our understanding of host-pathogen interactions. However, studies using these models have been limited by the fact that they lack the complexity of the human body. Therefore, recent efforts that allow tissue architecture to be mimicked during culture have included the development of methods and technology that incorporate tissue structure, cellular composition, and efficient long-term culture. These advances have opened the door for the study of pathogens that previously could not be cultured and for the study of pathophysiological properties of infection that could not be easily elucidated using traditional culture models. Here we discuss the latest studies using organoids and engineering technology that have been developed and applied to the study of host-pathogen interactions in mucosal tissues.
在过去的几十年中，细胞培养系统极大地扩展了我们对宿主-病原体相互作用的理解。然而，这些模型的研究受到了它们缺乏人体复杂性的限制。因此，最近的一些努力使得在培养过程中能够模拟组织架构，包括开发方法和技术，将组织结构、细胞组成和高效的长期培养结合起来。这些进展为研究以前无法培养的病原体以及研究使用传统培养模型难以阐明的感染病理生理特性打开了大门。在这里，我们讨论了使用类器官和工程技术的最新研究进展，这些研究已经被开发并应用于研究粘膜组织中的宿主-病原体相互作用。
