Faculty of Education, Gifu University, Gifu, 501-1193, Japan.
Okinawa Institute of Science and Technology Graduate University (OIST), Onna, Okinawa, 904-0495, Japan.
Sci Rep. 2019 Mar 8;9(1):4002. doi: 10.1038/s41598-019-40509-6.
The mosquito, Aedes baisasi, which inhabits brackish mangrove swamps, is known to feed on fish. However, its host assemblage has not been investigated at the species level. We amplified and sequenced the cytochrome oxidase subunit I barcoding regions as well as some other regions from blood-fed females to identify host assemblages in the natural populations from four islands in the Ryukyu Archipelago. Hosts were identified from 230 females. We identified 15 host fish species belonging to eight families and four orders. Contrary to expectations from previous observations, mudskippers were detected from only 3% of blood-engorged females. The dominant host was a four-eyed sleeper, Bostrychus sinensis (Butidae, Gobiiformes), in Iriomote-jima Island (61%), while it was a snake eel, Pisodonophis boro (Ophichthidae, Anguilliformes), in Amami-oshima and Okinawa-jima islands (78% and 79%, respectively). Most of the identified hosts were known as air-breathing or amphibious fishes that inhabit mangroves or lagoons. Our results suggest that A. baisasi females locate the bloodmeal hosts within the mangrove forests and sometimes in the adjacent lagoons and land on the surface of available amphibious or other air-breathing fishes exposed in the air to feed on their blood.
栖息在咸淡水红树林沼泽中的伊蚊（Aedes baisasi）已知以鱼类为食。然而，其宿主组合尚未在物种水平上进行调查。我们扩增并测序了来自冲绳群岛四个岛屿的自然种群中吸血雌蚊的细胞色素氧化酶亚单位 I 条形码区域和其他一些区域，以鉴定其宿主组合。从 230 只雌性蚊子中鉴定出了 15 种宿主鱼类，分属于 8 个科和 4 个目。与之前观察到的结果相反，泥鯭仅在 3%的吸血雌蚊中被发现。在伊良部岛（Iriomote-jima），四眼睡鲨（Bostrychus sinensis，Butidae，Gobiiformes）是主要的宿主（占 61%），而在奄美大岛（Amami-oshima）和冲绳岛（Okinawa-jima），蛇鳗（Pisodonophis boro，Ophichthidae，Anguilliformes）是主要的宿主（分别占 78%和 79%）。大多数被鉴定的宿主都是栖息在红树林或泻湖中的呼吸空气或两栖鱼类。我们的结果表明，A. baisasi 雌蚊在红树林内寻找血餐宿主，有时也在相邻的泻湖和两栖或其他呼吸空气的鱼类暴露在空气中的表面降落以吸食其血液。
