Bourhia Mohammed, Haj Said Amal Ait, Chaanoun Ayoub, El Gueddari Fatiha, Naamane Abderrahim, Benbacer Laila, Khlil Naima
Laboratory of Chemistry-Biochemistry, Environment, Nutrition, and Health, Faculty of Medicine and Pharmacy of Casablanca, Hassan II University, 19 rue Tarik Ibn Ziad, Casablanca, Morocco.
Laboratory of Pharmacognosy, Faculty of Medicine and Pharmacy of Casablanca, Hassan II University, 19 rue Tarik Ibn Ziad, Casablanca, Morocco.
J Toxicol. 2019 Feb 3;2019:8203832. doi: 10.1155/2019/8203832. eCollection 2019.
(A) is a wild species of Aristolochiaceae family; its roots are used by Moroccan people against cancer for many years ago. The objective of the study was to investigate the phytochemical screening, acute and subacute toxicity of roots growing in the north of Morocco. Qualitative and quantitative analyses of roots were performed using standard methods; the acute toxicity of the root extract of the studied plant was assessed in mice by gavage of single doses of 1, 2, and 4 g/kg body weight for 14 days; by the time the subacute toxicity was done using repeated doses 1, 1.5, and 2 g/kg/day for 28 days. Histological changes and biochemical parameters as markers of kidney and liver function were evaluated. The results of phytochemical screening showed the presence of polyphenols, tannins, alkaloids, flavonoids, saponins, and the absence of anthraquinones, sterols, and terpenes. The results of acute toxicity showed the absence of mortality and signs of toxicity in groups treated with 1 and 2 g/kg; however, the clinical signs of toxicity were important and the rate of mortality was estimated at 16 % in the group treated with 4 g/kg. The results of subacute toxicity showed several changes of serum parameters registered in groups treated with 1.5 and 2 g/kg/day, respectively. The results showed also the absence of histological injuries in groups treated with 1 and 1.5 g/kg/day; meanwhile, the histological alterations were remarkable in treated group with the highest dose administered of 2 g/kg/day. The outcome of this work showed that the roots' extract of the studied plant was toxic in mice with repeated doses, but no toxic effect was observed with a single dose under 4g/kg.
(A)是马兜铃科的一种野生植物；多年来摩洛哥人一直使用其根部抗癌。本研究的目的是调查摩洛哥北部生长的该植物根部的植物化学筛选、急性和亚急性毒性。采用标准方法对根部进行定性和定量分析；通过对小鼠灌胃单剂量1、2和4 g/kg体重，持续14天来评估所研究植物根部提取物的急性毒性；通过重复剂量1、1.5和2 g/kg/天，持续28天来进行亚急性毒性实验。评估了作为肾脏和肝功能标志物的组织学变化和生化参数。植物化学筛选结果显示存在多酚、单宁、生物碱、黄酮类、皂苷，不存在蒽醌、甾醇和萜类。急性毒性结果显示，用1和2 g/kg处理的组没有死亡和毒性迹象；然而，用4 g/kg处理的组毒性临床症状明显，死亡率估计为16%。亚急性毒性结果显示，分别用1.5和2 g/kg/天处理的组血清参数有若干变化。结果还显示，用1和1.5 g/kg/天处理的组没有组织学损伤；同时，给予最高剂量2 g/kg/天的处理组组织学改变显著。这项工作的结果表明，所研究植物的根部提取物在重复给药时对小鼠有毒，但在4 g/kg以下的单剂量时未观察到毒性作用。
